中药治疗大鼠卵巢过度刺激综合征模型VEGF和IL-6的相关性研究

Study on the Correlation between VEGF,IL-6 and Ovarian Hyperstimulation Syndrome

目的:观察自拟方剂参芪利水汤治疗大鼠卵巢过度刺激综合征(OHSS)模型中血管内皮生长因子(VEGF)和白细胞介素-6(IL-6)的变化,探讨其与OHSS的相关性,为临床上进一步治疗OHSS提供实验学基础。方法:选择未成年雌性Wistar大鼠30只,皮下注射孕马血清促性腺激素(PMSG)和人绒毛膜促性腺激素(HCG)制成OHSS模型,随机分为实验组、对照组和空白对照组三组,实验组采用参芪利水汤灌胃,对照组用吲哚美辛灌胃,空白对照组为空白对照。1周后用ELISA法检测各组血中VEGF和IL-6的含量,采用免疫组化方法观察大鼠卵巢黄体VEGF和IL-6的表达。结果:实验组和对照组血清中VEGF、血浆IL-6含量及卵巢黄体和子宫内膜中VEGF和IL-6表达在统计学上有显著性差异(P<0.05)。结论:中药治疗OHSS时可明显降低VEGF和IL-6的含量。

Objective： To detect the changes of VEGF and IL-6 in treating rats with ovarian hyperstimulation syndrome （OHSS）, so as to explore a new way to cure OHSS in clinics. Methods： Thirty rats were divided randomly into three groups： experimental group, control group, and blank control group. ELISA method was applied to detect the level of VEGF and IL-6 in blood. Immunohistochemical method was applied to detect the expression of VEGF and IL-6 in uterus and ovary. Results ： In the content and expression of VEGF and IL-6, there was no significant difference between experimental group and control group in statistics （ P 〉 0.05 ） . But the content and expression of VEGF and IL-6 in experimental group and control group were significantly less or weak than that in blank control group. Conclusion： Chinese traditional medicine can decrease the level of VEGF and IL-6 in treating OHSS rats.