海尔福口服液对O_2^-清除率的实验研究

Experimental study of Haier oral liquid on clearing

目的:探讨海尔福口服液对超氧阴离子自由基(O2-)的清除效果及其机理。方法:用AP-TEMED系统比色法测定海尔福口服液对O2-的清除率,并与维生素C及金银花提取液作对比分析。结果:海尔福口服液(内含金银花生药0.1g/ml)、金银花水提取液(含生药0.1g/ml)、维生素C(浓度1.0mg/ml)对O2-的清除率分别是(92.71±2.79)、(81.15±3.81)、(92.67±5.46),加样量分别为1.0、0.5、0.25、0.125ml时,它们的相关系数分别为0.9697、0.9959、0.9988,有直线相关关系;三种存放时间不同的金银花提取液对O2-的清除率分别是(81.15±3.81)、(87.70±1.41)、(37.94±11.79),差异显著(P<0.05和<0.01);海尔福口服液生产过程中的不同阶段对O2-的清除率无显著性差异(P>0.05);海尔福口服液的其余主要原料如茯苓、甘草等对O2-的清除率均较低。结论:海尔福口服液及其主要原料之一金银花提取液在体外对O2-均有明显的清除作用,其效果与浓度为1.0mg/ml的维生素C溶液接近;金银花原料短期存放功效无明显变化,长期存放对O2-的清除率明显降低;海尔福口服液在生产过程中的不同阶段对O2-的清除率无明显变化。

Objective：To study the effection of clearance of O2^- by Hierfu oral liquid and its mechanism. Methods：To determmine clearance of O2^- by Haierfu oral liquid with AP-TEMED systemic colorimetry,and compare with vitamine C and Jinyinhua extraction. Results： Haierfu oral liquid in which 1.0ml there is 0. 1g Jinyinhua,Jinyinhua extraction in which 1.0ml there is 0. 1g flash medicine,Vitamine C which concentration is 1.0mg/ ml, their clearance of O2^- is （92.71±2.79） % ,（81.15±3.81）% ,（92.67±5.46）% ,added quality is 1.0ml, 0.5ml, 0.25ml and 0. 125ml. Clearance of O2^- of three kind of Jinyinhua with different restore time is （81.15±3.81）%,（87.70±1.41）% and（ 37.94±11.79）%,（P〈0.05 and P〈 0.01 ）, there is significant difference. Clearance of O2^- by Haierfu oral liquid with differente extracted period no significant difference among them（P 〉0.05）. Clearance of O2^- are all lower which clear by Haierfu oral liquid and its main material Fuling and Gancao. Conclusions： Haierfu oral liquid and its main material Jinyinhua extraction have the function on clearing O2^- , which close to vitamine C （1.0mg/ml）. No signifiaeant change of Jinyinhua with short store, but decrease severely with long store, no significant change of clearance of O2^- by Haierfu oral liquid during its product period.