发育小鼠胰腺胃泌素免疫反应细胞的形态及数量变化

Observation of Gastrin Immunoreactive Cells in the Pancreas of Developing Mouse

目的观察小鼠胰腺内胃泌素免疫反应细胞(G-IR细胞)的个体发生、分布、形态及数量变化。方法免疫组织化学技术。结果小鼠胰腺中G-IR细胞最早出现于胚胎第12d,至胚胎第18d前分布于外分泌部及胰岛内;此后直至成年,仅见于胰岛内。G-IR细胞的总数胚胎期以第15d组最多,随后数量逐渐减少,至第17d组降至最低;此后至出生直至成年,数量逐渐增加。强阳性G-IR细胞数量以胚胎第15d组最多,出生后第15d至成年,胰腺内无强阳性细胞。G-IR细胞形态多样,可见免疫反应阳性的细胞突起伸至其它细胞之间。结论小鼠胰腺中G-IR细胞发生、分布及数量变化规律有别于其它种属。胃泌素在胚胎期小鼠胰腺内的表达,提示其可能与胰腺早期发育有关。

Objective To observe the ontogeny, distribution, morphology and quantitative changes of gastrin immunoreactive（G-IR） cells in the pancreas during the development of mouse. Methods Immunohistochemical method was used. Results G-IR cells could be found in the mouse pancreas as early as ED 12, distributed in the pancreatic islets and the exocrine part before ED 18, but limited to the islets from ED 18 to day 45 after birth. Total amount of G-IR cells showed a peak at ED 15 before birth and gradually decreased afterwards, down to the bottom at ED 17. The quantity of G-IR cells began to increase again from ED 18 to day 45 after birth. The number of strong positive staining cells showed a peak at ED 15 and then decreased with development, but with no strong positive from day 15 after birth to mature. Some G-IR immunoreactive positive processes could be seen extending between the adjacent cells. Conclusion The ontogeny and distribution of G-IR cells in the mouse pancreas were different from the other species, the expression of gastrin in the embryo suggests that the gastrin might have a role in the early development of the pancreas.