温度、pH对胡子鲶主要消化酶活性的影响

Effect of Temperature and pH on the Activity of Major Digestive Enzymesin Claris fuscus Lacepede

研究胡子鲶胃、前肠、中肠、肝胰脏四个部位的主要消化酶（蛋白酶、淀粉酶、脂肪酶）活性以及温度、pH对其的影响．结果表明：在四个部位中，蛋白酶活力由高到低依次为：胃〉前肠〉肝胰脏〉中肠；淀粉酶活力由高到低依次为：肝胰脏〉中肠〉胃〉前肠；脂肪酶活力由高到低依次为：前肠〉中肠〉胃〉肝胰脏．胃蛋白酶活性最高，其适宜温度为30-35℃。适宜pH为5．5；肝胰脏淀粉酶活性最高，其适宜温度为25~35℃。适宜pH为6．0～7．0；前肠脂肪酶活性最高，其适宜温度为35-40℃，适宜pH为7．0～7．5．

Effect of temperature and pH on the activity of major digestive enzymes （protease, amylase, Lipase ） in stomach, foregut, midgut, hepatopancreas of Claris fuscus lacepede were studied in this experiment. The result indicates that the activity of protease from high to low： stomach〉foregut〉 hepatopancreas〉midgut;the activity of amylase from high to low. hepatopancreas〉midgut〉stomach 〉foregut; the activity of lipase from high to low. foregut〉midgut〉 stomach〉hepatopancreas. The optimum temperature for protease of stomach is 30-35℃, for amylase of hepatopancreas is 25-35 ℃ ,and for lipase of intestine is 35-40℃. The optimum pH for protease is 5.5 in stomach,for amylase is 6.0-7.0 in hepatopancreas,for lipase is 7.0-7.5 in foregut.