广西南部某市周边农场奶牛隐孢子虫感染调查

Investigation on cow infection with Cryptosporidium parvum in south areas of Guangxi

目的调查广西南部某市奶牛隐孢子虫感染情况，为防治该病提供依据。方法粪样经水醚沉淀法和蔗糖离心浮聚法处理，涂片后用改良Ziehl—Neelsen抗酸染色法染色镜检，计算感染率和感染度。结果在7个奶牛场中检查了429头奶牛，隐孢子虫平均感染率为8．16％，14月龄犊牛和1岁龄以上奶牛感染率分别为7．69％（5／65）和8．24％（30／364）。结论广西奶牛隐孢子虫感染比较常见，居民可因摄入受病牛粪便污染的食品或水而受感染。控制该病的传染源和传播途径十分重要。

Objective To investigate the state of cow infected with Cryptosporidium parvum and probe the preventive strategy. Methods Fresh stool samples of cow were concentrated with water-ether sedimentation and sucrose centrifugal flotation method. The smears were stained with modified Ziehl-Neelsen method and observed under oil immersion lens. Results Totally 429 stool samples from 7 cow farms were checked and 35 samples were found to be positive with a infection rate of 8.16%. The infection rate of group 1--4 month cows was 7.69 （5/65）, and the infection rate of group above 1 year was 8.24% （21/30）. There was no significant difference between two groups （P〉0.05）. Conclusion Cows infected with Cryptosporidium parvum were common in guangxi. The residents may be infected with Cryptosporidium parvum through contacting with food and water contaminated. The effective measures be taken to control the source of infection.