孕早期和待产孕妇血清蛋白质含量分析

Analysis on serum protein level of pregnant women in the early trimester of pregnancy and the parturicent women

目的调查分析孕妇孕早期和待产时血清蛋白质含量,探讨建立孕妇血清蛋白参考范围的必要性。方法奥林巴斯全自动生化分析仪定量检测1 082例孕早期孕妇和963例待产孕妇血清中总蛋白(双缩脲法)、清蛋白含量(溴甲酚绿法)。统计各组均值(x)与标准差(s),以2s建立相应的参考范围。单一样本t检验与健康成人血清蛋白含量进行统计学比较。结果孕早期总蛋白x为73.90 g/L,s为4.60,参考范围为64.90～82.90 g/L;清蛋白x为42.30 g/L,s为4.39,参考范围为33.70～50.00 g/L。待产时总蛋白x为64.90 g/L,s为6.06,参考范围为53.00～76.80 g/L;清蛋白x为34.20 g/L,s为4.29,参考范围为25.80～42.60 g/L。与健康成人血清总蛋白(63.00～83.00 g/L)、清蛋白(35.00～50.00 g/L)[1]进行单一样本t检验,孕早期清蛋白与健康成人清蛋白含量差异无统计学意义,孕早期总蛋白以及待产时血清总蛋白和清蛋白与健康成人血清蛋白含量差异均有统计学意义。结论在孕早期,清蛋白含量与健康成人清蛋白差异无统计学意义,总蛋白含量统计学上虽有差异,但数值上相差不大,无实际临床价值,可以通用健康成人参考范围。待产时,孕妇血清蛋白含量与健康成人血清蛋白含量在统计学和临床上差异有统计学意义,有必要另行建立其相应的参考范围。

Objective To investigate the serum protein level of pregnant women in the early trimester of pregnancy and and the parturicent women,and explore the necessity in establishing the reference range of serum proteins in pregnant women. Methods Olympus automatic biochemicalanalyzer was applied to quantitatively measuring serum level of total protein （biurea method） and albumin （bromcresol green method） in1082 pregnant women in the early trimester of pregnancy and 963 parturicent women. The results were shown as mean±s,and the reference range was established. One-sample t test was carried out. Results Pregnant women in the early trimester of pregnancy：total protein level （73.90±4. 60） g/L,reference range （64. 90--82.90 g/L） ; albumin level （42.30±6.06） g/L,reference range （33.70--50.00 g/L）. The parturicent women., total protein level （64. 90 ± 4. 60） g/L, reference range （53.00--76, 80 g/L）; albumin level （34.20±4.29） g/L,reference range （25.80--42.60 g/L）. One-sample t test showed there is no statistical difference in albumin level between early trimester of pregnancy group and healthy control group,whereas there was between parturicent women and healthy controls. The difference in total protein level was significant in pregnant women in the early trimester of pregnancy and the parturicent women between compared with healthy control group. Conclusion In the early trimester of pregnancy, there is no significant difference in the serum Alb compared with health adutts; althought the difference of the serum TP is statistically significant,there is no clinical applicable value; therefore the reference ranges of serum proteins in healthy adults can be used in pregnant women in the early trimester of pregnancy. As the parturient women are concerned, there are significent differences of the serum proteins in statistics and clinic compared with those of healthy adults, so it is necessary to establish their reference ranges.