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宫颈癌中H19基因的特异表达模式及印记缺失分析 被引量：4

Analysis of the specific-expressing and loss of imprintmg of H19 gene in uterine cervical cancer
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摘要 目的探讨H19等位基因在宫颈癌中特异性表达的模式,H19基因的基因组印记缺失与宫颈癌两者之间的关系。方法结合PCR和限制性片段长度多态性分析(RFLP)技术以及DNA甲基化检测技术分析我院收集的宫颈癌标本H19等位基因进行杂合子筛选,并进一步研究基因组印记缺失与宫颈癌两者之间的关系。结果在36例宫颈癌中筛选出16例杂合子现象的标本,在这16例杂合子标本中,有7例发生了基因组印记缺失;H19基因发生基因组缺失现象与其启动子区域的高度去甲基化正相关。结论H19基因的基因组印记缺失可能和宫颈癌的发生有着高度的相关性。 Objective To investigate status of H19 gene imprinting and methylation in H19＇s CBS6 region in uterine cervical cancer.Methods With PCR-RFLP and bisulfite treatment of genomic DNA,it was analyzed of H19 gene specific-ex-pressing panel and methylation at CpG sites in H19＇s CBS6 region in 36 uterine cervical cancer samples in our hospital.Results It was identified that LOI of H19 gene in 7 uterine cervical cancer samples among 36 uterine cervical cancer samples,and that LOI of H19 gene could be linked to hypomethylation but not hypermethylation.Conclusion The frequent LOI of H19 gene might play an important role in the development of uterine cervical cancer.
作者 邱剑萍
机构地区 江苏省苏州市市立医院妇产科
出处 《中国现代医药杂志》 2008年第11期31-33,共3页 Modern Medicine Journal of China
关键词 H19基因 基因组印记缺失 DNA甲基化 宫颈癌 H19 gene Loss of Imprinting DNA methylation Uterine cervical cancer
分类号 R737.33 [医药卫生—肿瘤]
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参考文献10

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同被引文献50

  • 1王月玲,杨新园,李旭.印记基因H19和胰岛素样生长因子Ⅱ在人子宫颈癌组织中印记缺失及意义[J].西安交通大学学报（医学版）,2006,27(4):372-376. 被引量：6
  • 2胡振富,李薇,宋艳斌,李晓平.病理性瘢痕中IGF2基因的表达及意义[J].广东医学,2007,28(4):530-531. 被引量：6
  • 3Franco M M, Prickett A R, Oakey R J. The Role of CCCTC - Binding Factor (CTCF) in Genomic Imprint- ing, Development, and Reproduction [ J ]. Biol Reprod, 2014, 91 (5): 125. doi: 10. 1095/biolreprod. 114. 122945. Epub 2014 Oct 8.
  • 4Jouvenot Y, Poirier F, Jami J, et al. Bialleie transcrip- tion of IGF2 and H19 in individual cells suggests a post transcriptional contribution to genomic imprinting [ J ]. Current Biology, 1999, 9(20): 1199- 1202.
  • 5Takai D, Gonzales F A, Tsai Y C, et al. Large scale mapping of methyleytosines in CTCF - binding sites in the human H19 promoter and aberrant hypomethylation in hu- man bladder cancer [ J]. Hum Mol Genet, 2001, 10 (23) : 2619 -2626.
  • 6Bell A C, Felsenfeld G. Methylation of a CTCF dependent boundary controls imprinted expression of the Ig[2 gene [J]. Nature, 2000, 405(6785): 482 -485.
  • 7Nakagawa H, Chadwick R B, Peltomaki P, et al. Loss of imprinting of the insulin - like growth factor 11 gene oc- curs by biallelic methylation in a core region of H19 - as- sociated CTCF - binding sites in coloreetal cancer [ J ]. Proc Natl Acad Sci USA, 2001, 98(2) : 591 -596.
  • 8Cui H, Niemitz E L, Ravenel J D, et al. Loss of imprint- ing of insulin - like growth factor - II in Wilms' tumor commonly involves altered methylation but not mutations of CTCF or its binding site[J]. Cancer Res, 2001, 61 (13) : 4947 -4950.
  • 9Ulaner G A, Vu T H, Li T, et al. Loss of imprinting of.IGF2 and H19 in osteosarcoma is accompanied by recipro- cal methylation changes of a CTCF - binding site [ J ]. Hum Mol Genet, 2003, 12(5) : 535 -549.
  • 10Cui H, Onyango P, Brandenburg S, et al. Loss of im- printing in colorectal cancer linked to hypomethylation of H19 and IGF2[J]. Cancer Res, 2002, 62(22) : 6442 - 6446.

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中国现代医药杂志
2008年 第11期

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