汉防己甲素逆转人肝癌耐药细胞株多药耐药性的研究

Reversal effect of Tetredrine （TTD） on human hepatocellular carcinoma （HCC） multidrug-resistant cell line （Hep-3B/ADM）

目的研究汉防己甲素对人肝癌多药耐药细胞株Hep-3B／ADM耐药性的逆转作用及其机制。方法通过阿霉素（ADM）浓度梯度递增诱导法，建立人肝癌多药耐药细胞株Hep-3B／ADM。MTT法检测细胞对化学疗法药物的敏感性；流式细胞仪检测细胞表面多药耐药基因表达产物P-170及分析细胞内若丹明123染剂相对荧光强度。结果汉防己甲素（0．10～1．00μmol／L）可逆转肝癌耐药细胞的耐药性；汉防己甲素明显降低细胞表面P-170的表达。结论汉防己甲素具有增强阿霉素对Hep-3B／ADM细胞的毒性作用，其作用机制与逆转多药耐药基因有关。

Objective To investigate reversal effect on Hep-3B/ADM by TTD and its mechanism. Methods An adriamyein-resistant human HCC cell line （Hep-3B/ADM） was established, through exposure to gradually increased concentration of ADM. Drug sensitivity was measured by MTF. Flow cytometry （FCM） was performed to assess the expression of P-170 and the fluorescene intensity of Rh13 in the cells. Results ITD（0.10- 1. 00 μmol/L） can reverse multidrug-resistant of Hep-3B/ADM. TTD decreased the expression of P-170 in the cells significantly. Conclusion TTD significantly enhancs ADM-induced cytotoxicity. The mechanism is mediated by inhibiting multidrug-resistant （ MDR1 ）.