原位杂交检测pal-1 mRNA在C.elegans野生型和突变体早期胚胎中的分布

The Distribution of pal-1 mRNA in C.elegans Wild-type and Mutants Early Embryo Via in situ Hybridization

Caenorhabditis elegans是发育生物学中的重要模式生物,许多基因参与C.elegans的细胞命运决定和细胞谱系发育.par基因的活性影响C.elegans胚胎前后轴的极性,其功能缺失会使胚胎的第一次分裂丧失前后轴的不对称性,导致一些母体提供的发育调控因子不能在特定的胚胎细胞中准确定位,从而改变胚胎细胞发育命运.pal-1是C.elegans早期胚胎发育中决定细胞命运的重要基因,它决定体细胞的性质,也是转录因子,调控着后续基因的表达,凡含有该基因表达的细胞发育成体细胞.调控PAL-1表达的基因有许多,本文通过原位杂交检测pal-1 mRNA在C.elegans野生型和par-5、par-6、gld-1突变体早期胚胎中的分布,来探讨par-5、par-6和gld-1基因的活性在胚胎发育早期对pal-1 mRNA的影响.实验结果表明,pal-1mRNA在野生型早期胚胎中有明显极性,而pal-1mRNA分布的不对称性在上述基因缺失的情况下被破坏,这些基因与pal-1mRNA的不对称分布直接相关.

C. elegans is an important model animal in developmental biology. A lot of genes involve in C. elegans cen fate determination and linage development. The activities of par genes are responsible for establishing AP axis. Loss of par function results in the loss ofAP asynmaetry during the first two embryonic cell divisions, a failure to restrict developmental regulators to specific embryonic cells and abnormal cell fate patterning, pal-1 is an important gene which determines cell fate in C. elegans early embryo devdopment. It is a transcription factor, which regulates the expression of downstream genes to determine the characteristic of somatic cell. A lot of genes regulate the expression of pal-1. The distribution of pal-1 mRNA was detected in C. elegans early embryo of wild type and par-5, par-6 and gld-I mutants via in situ hybridization. The results showed that the asymmetric distribution ofpal-lmRNA was obvious in wide type 2-cen and 4-cell embryo; the asymmetric location of pal-1 mRNA was lost when par-5 ,par-6 and gld-1 was absent. These results indicated that these genes were directly related to the asymmetric location of pal-1 mRNA in wild type embryo, par-5 ,par-6 and gld-1 affected the pal-1 mRNA distribution in C. elegans early embryo.