β-细辛醚对缺血/再灌注损伤心肌细胞的保护作用

Protective Effects of β-asarone against Myocardial Ischemia/Reperfusion Injury in Cultured Cardiac Myocytes

目的观察β-细辛醚对缺血/再灌注损伤(MI/RI)心肌细胞的保护作用。方法体外培养原代乳鼠心肌细胞,采用连二亚硫酸钠(Na2S2O4)诱导心肌细胞MI/RI,测定细胞存活率(MTT法)和细胞培养液中乳酸脱氢酶(LDH)、肌酸激酶(CK)的含量。结果在MI/RI模型中,β-细辛醚能显著提高细胞存活率,明显降低培养液中LDH、CK的含量。结论β-细辛醚对MI/RI心肌细胞具有显著的保护作用。

Objective To study the protective effects of β-asarone against the myocardial ischemia/ reperfusion injury （MI/RI） in cultured cardiac myocytes. Methods MI/RI model was set up with Na2S2O4, which make use of rat cardiac myocytes. The viability of cardiac myocytes （using MTT method）, dehydrogenase （LDH, CK） activitices in the medium were measured. Results In MI/RI model, β-asarone can obviously advance the viability of cardiac myocytes, and decrease the dehydrogenase （LDH, CK） activitices in the medium. Conclusion β-asarone showed protective effects against the myocardial ischemia/reperfusion injury in cultured cardiac myocytes.