高效液相色谱法测定银蒲解毒片中蒙花苷含量

Content Determination of Buddleoside in Compound Yinpujiedu Tablet by HPLC

目的建立银蒲解毒片中蒙花苷的含量测定方法。方法采用高效液相色谱法,AgilentEclipseXDB-C18色谱柱(4.6mm×250mm,5μm),流动相为甲醇-4%冰醋酸水溶液(47∶53),检测波长334nm。结果蒙花苷线性范围为2.24～44.8μg(r=0.9991);平均回收率为97.8%,RSD=1.52%。结论方法可靠,简单可行,为控制银蒲解毒片的内在质量提供了依据。

Objective To establish a method for content determination of Buddleoside in Compound Yinpujiedu Tablet. Method HPLC method, Agilent Eclipse XDB-C18 column （4.6 mm×250 mm, 5 μm） was used, mobile phase was methanol-4% glacial acetic acid （47 ： 53）, and the detecting wavelength was set at 334 nm. Result The standard curve was linear within the range of 2.24-44.8μg. The average recovery and RSD were 97.8% and 1.52%, respectively. Conclusion This method is accurate, reliable, and can provide a scientic index for quality control of Compound Yinpujiedu Tablet.