正交试验优选昆布中褐藻酸的提取工艺

Optimization of Alginic Acid in Laminaria japonica Aresch by Orthogonal Test

目的优选昆布中褐藻酸的最佳提取工艺。方法采用正交设计L9(34),考察溶剂量、提取时间、提取温度对褐藻酸含量和得率的影响,并进行方法学考察。结果最佳制备工艺为A1B1C2,即药材加入30倍量1%Na2CO3溶液,60℃搅拌提取1h。结论提取温度为最重要的影响因素,高于60℃时褐藻酸含量会明显下降。优选出的最佳提取工艺是合理的。

Objective To optimize the extraction technology of alginic acid in Laminaria japonica Aresch. Methods The influence of the amount of solvent, the extraction time and temperature （3 factors） on the alginic acid content and yield was investigated by orthogonal test L9（3^4）, and carried on to inspect the methodology. Result The optimum extraction condition was A1B1C2, that was adding 30 times solvent （1%Na2CO3）, whisk extracting for one hour at 60 ℃. Conclusion The temperature is the most important factor. The content of alginic acid decrease obviously at above 60 ℃. The optimum extraction technology is reasonable.