反相高效液相色谱法同时检测片剂中的头孢呋辛酯及奥硝唑(英文)

Simultaneous determination of cefuroxime axetil and ornidazole in tablet dosage form using reversed-phase high performance liquid chromatography

A simple,accurate and sensitive reversed-phase high performance liquid chromatographic(RP-HPLC)method for the simultaneous determination of cefuroxime axetil and ornidazole in combined tablet dosage form has been developed.The method was performed with a HiQ-SiL C18 column(250 mm×4.6 mm)and photodiode array(PDA)detector,using 0.01 mol/L potassium dihydrogen orthophosphate-methanol(56∶44,v/v)as the mobile phase and tinidazole as the internal standard.Beer's law obeys in the concentration ranges of 5-25 μg/mL and 10-50 μg/mL for cefuroxime axetil and ornidazole,respectively.The method has been successfully validated statistically and applied for the analysis of the drugs in pharmaceutical formulation.

A simple, accurate and sensitive reversed-phase high performance liquid chromatographic （RP- HPLC ） method for the simultaneous determination of cefuroxime axetil and ornidazole in combined tablet dosage form has been developed. The method was performed with a HiQ-SiL C18 column （250 mm ×4.5 mm） and photodiode array （PDA） detector, using 0.01 mol/L potassium dihydrogen orthophosphate-methanol （55：44, v/v） as the mobile phase and tinidazole as the internal standard. Beer＇s law obeys in the concentration ranges of 5 -25 μg/mL and 10 -50 μg/mL for cefuroxime axetil and ornidazole, respectively. The method has been successfully validated statistically and applied for the analysis of the drugs in pharmaceutical formulation.