直链淀粉手性固定相高效液相色谱法拆分比索洛尔对映体

Chiral separation of bisoprolol using high performance liquid chromatography with amylose chiral stationary phase

采用直链淀粉手性固定相高效液相色谱法在正相条件下直接拆分了比索洛尔对映异构体。分别以异丙醇、乙醇为有机改性剂,考察了流动相的组成与配比、流速及柱温等因素对比索洛尔对映体分离的影响。确定了比索洛尔对映体的最佳拆分条件:流动相正己烷-乙醇-二乙胺(体积比为88∶12∶0.1),流速0.6mL/min,检测波长270nm,柱温20℃。该方法可快捷、简便地拆分比索洛尔对映体。

A high performance liquid chromatographic（HPLC）method using amylase chiral stationary phase for the separation of bisoprolol enantiomers has been developed.Tris（3,5-dimethylphenyl carbamate）amylase chiral stationary phase was used in the normal-phase mode.The effects of the concentration of organic solvents（iso-propanol and ethanol）,flow rate and column temperature on the enantiomer separation were studied systematically.The best separation was obtained with hexane-ethanol-diethyl amine（88∶12∶0.1,v/v/v）as mobile phase at 20 ℃.The optimal flow rate was 0.6 mL/min.The detection wavelength was set at 270 nm.By using this method,the enantiomers of bisoprolol can be separated quickly and easily.