差示分光光度法测定复方刺梨合剂中总黄酮的含量

Determination of total flavonoids in Fufang Cili mixtare by Differential Spectrophotometry

目的测定复方刺梨合剂中的总黄酮含量；方法采用差示分光光度法，在392nm波长下样品不经分离直接测定；结果 线性范围5．06-50．60μg·mL^-1，回归方程A=5．84×10^-4＋0．01321C．r=0．9996，平均回收率99．59％，RSD=1．49％。结论所建方法快速、简便、准确。适用于复方刺梨合剂的质量控制。

OBJECTIVE To determine total flavonids in Fufang Cili mixture. METHODS Differential spectrophotometry was applied without prior separation at 392nm. RESULTS The average recovery and the relative standard deviation of flavonoids were 99.59% and 1.49%, the equation was A= 5.84 × 10^-4 ＋ 0.01321C, r = 0. 9996, the linear range is between 5.06 - 50.60μg· mL^- 1. CONCLUSION This method is simple, quick＇and accurate. It can become a control method for Fufang Cili mixture.