超声波照射激活亚甲基蓝(MB)声动力损伤牛血清白蛋白(BSA)

Sonodynamic Damage of Bovine Serum Albumin(BSA) under Ultrasonic Irradiation in the Presence of Methylene Blue(MB)

应用紫外-可见(UV-Vis)光谱和荧光光谱研究了超声波激活亚甲基蓝(MB)对牛血清白蛋白(BSA)分子的损伤,考察了超声波照射时间,MB浓度,溶液酸度对BSA分子损伤的影响。结果表明,在一定条件下,BSA分子的损伤程度随着超声波照射时间,MB浓度和pH值增加而增加,即UV-Vis光谱表现出越来越明显的增色效应,荧光光谱表现出越来越明显的猝灭现象。探讨了超声波激活MB对BSA分子损伤的可能机理。

The damage of bovine serum albumin （BSA） molecules under the action of methylene blue （MB） activated by ultrasonic irradiation was detected by UV-Vis spectroscopy and fluorescence spectroscopy, and influencing factors such as ultrasonic irradiation time, MB concentration and pH value on the damage of BSA molecules were also investigated. The results show that under certain conditions, the damage of BSA molecules was enhanced with the increases of the ultrasonic irradiation time, MB concentration, and pH value, that is, UV-Vis spectra showed more and more obvious hyperchromic effect and fluorescence spectra exhibited more and more obvious quenching effect. In addition, the possible mechanism of sonodynamic damage of BSA molecules from MB activated by ultrasound was also discussed in this work. The result has certain significance for the application of photosensitive drugs to sonodynamic therapy（SDT）.