CdS／PPA纳米溶胶荧光探针同步荧光光度法测定水溶液中牛血清白蛋白

Determination of BSA in Water Solution by Synchronous Fluorescence Spectrometry with CdS/PPA Nanoparticle Solution as Fluorescence Probe

在水溶液中合成了CdS纳米粒子，利用超声辐射下原位聚合方法合成了聚4-戊烯酸（PPA）包裹的CdS／PPA复合纳米溶胶。作为荧光探针，建立了同步荧光光度法测定牛血清蛋白的工作曲线△F=117．50＋29．55c（1×10^-6g／mL），线性范围为0．50×10^-6～30×10^-6g／mL，检出限为0．12×10^-6g／mL。对合成样品进行了测定，结果令人满意。

CdS nanoparticles were synthesized in water solution, the surface of CdS nanoparticles was modified with poly-4-pentenoic acid under ultrasonic irradiation. With CdS/PPA nanoparticle solution as fluorescence probe combined with synchronous fluorescence spectrometry, a method for determination of BSA in water solution was developed. The working curve for determination of BSA is AF = 117.50 ＋ 29.55c（ 1 × 10^ -6 g/mL）, the linear range is 0. 5 × 10^-6 -30 × 10 ^-6 g/mL, the limit of detection is 0. 12 × 10 ^-6 g/mL. In addition, the present method was applied to quantifying BSA in synthetic samples.