细胞免疫表型检测在急性淋巴细胞白血病诊断中的应用价值

The Application Value of Cell Immunity Phenotype Detection in the Diagnosis of Acute Lymphocyte Leukemia

目的:对26例初诊或化疗未缓解的急性淋巴细胞白血病(ALL)患者进行免疫表型检测分析。方法:采集白血病患者EDTA-K2抗凝外周血或骨髓液,用流式细胞术检测髓系、T系、B系、红系、巨系分化抗原及非特异性抗原。结果:ALL患者均表达淋巴系抗原,但有部分病例同时表达髓系抗原(CD13或CD33)。3例髓系淋系双表型杂合性白血病同时表达2个以上淋系和髓系抗原标志,1例T系、B系双克隆杂合性白血病细胞分别表达两种以上T系和B系抗原。结论:细胞免疫表型检测可弥补形态学的不足,提高分型正确性,结合细胞形态学检验能为急性淋巴细胞白血病的诊断、治疗、预后判定及基础研究提供可靠的依据。

Objective：To detect and analyze immunity phenotype of 26 acute lymphocyte leukemia （All） patients who have not been relieved after preliminary diagnosis or chem.Methods：To collect EDTA-K2 anticoagulation peripheral blood or bone marrow liquid of ALL patients and detect differentiation antigen of medullary system,T system,B line,red line,huge system and non-specificity antigen by using flow cytometry method.Results：ALL patients express systema lymphaticum antigen,but some patients express medullary system antigen CD13 or CD33 meanwhile.Three patients medullary system and systema lymphaticum who are double phenotype and heterozygosita express above two medullary system and systema lymphaticum antigen maker.One patient who has double clone heterozygosita leukemia cell that is T system and B line express above two kinds of T system and B line antigen separately.Conclusions：Cellimmunity phenotype detection could redeem the insufficient of morphology and raise the correctness of grouping and giving the reliable reason for diaguosis,treatment,prognosis assessment and foundation research of ALL combining the cell morphology inspection.