丙戊酸代谢酶中CYP2C9、UGT1A6的基因多态性分析方法的建立

An establishment of analysis of CYP2C9、UGT1A6 gene polymorphism on the metabolic enzyme of valproic acid

目的:研究药物代谢酶基因多态性对丙戊酸钠代谢的影响,并建立分析丙戊酸代谢酶中CYP2C9、UGT1A6的基因多态性的方法。方法:从人全血中抽取gDNA,设计引物扩增CYP2C9及UGT1A6,并运用限制性片段长度多态性(RFLP)技术的方法进行CYP2C9*2、CYP2C9*3、CYP2C9*4、CYP2C9*5、UGT1A6*2和UGT1A6*3的分型。结果:成功设计了引物用来扩增包含CYP2C9*3、CYP2C9*4、CYP2C9*5的片断,并测序验证。结论:建立了简单可靠的RFLP基因分型方法。

Objective： In order to study the effect of drug-metabolizing enzymes （DME） polymorphism on the metabolism of valproie acid （ VPA）, we set up a method to analyze the DME gene polymorphism. Methods：The GDNA were extracted from blood of human. The CYP2C9 and UGTIA6 gene were amplified with PCR. We used the restriction fragment length polymorphism （RFLP） to classify the CYP2C9 ＊ 2, CYP2C9 ＊ 3, CYP2C9 ＊4, CYP2C9 ＊ 5, UGTIA6＊ 2 and UGT1A6 ＊3 genotypes. Results：A successfttl design of the primers was used to contain expansion of CYP2C9＊ 3, CYP2C9 ＊ 4, CYP2C9 ＊ 5 of the dips, and sequencing and verification were performed. Conclusions：We established a simple and reliable RFLP method to group the UGT1A6 and CYP2C9.