摘要
为探析调节基因srrG对合成抗生素的直接影响以及在γ-丁酸内酯调节系统中的作用,通过构建破坏株、构建相应的重组质粒pRSET-B-srrG并在Escherichia coli BL21-Gold(DE3)pLysS中高效表达、与其他调节基因的破坏株联合培养,发现srrG破坏株无合成抗生素的能力,且在γ-丁酸内酯调节系统中具有一定作用.srrG在Streptomyces rochei合成抗生素中的功能具有重要的意义.
In order to detect the direct effect of srrG on the antibiotic biosynthesis and the function of srrG in γ-butyrolactone autoregulator-receptor system, construction of srrG disruptant and recombinant plasmid pRSET-B-srrG which would overexpress in Escherichia coli BL21-Gold （DE3） pLysS were applied, co-fermentation experiments were also applied. Disruption of srrG caused nonproduction of LC and LM, and gene srrG may have some special function in γ-butyrolactone regulatory system of S. rochei. These research results were meaningful in the function analysis of gene srrG in the antibiotic biosynthesis thoroughly.
出处
《宁夏工程技术》
CAS
2008年第4期332-336,共5页
Ningxia Engineering Technology
