可溶性喉鳞癌抗原诱导TAK细胞的免疫学特征

Immunologic features of the soluble tumor antigen activated killer (TAK) cell

从喉鳞癌患者的手术切除标本中提取的可溶性抗原具有免疫活性，在抗CD3单抗和rIL－2的协同作用下，能刺激外周血单核细胞（PBMC）增殖产生CD8＋细胞毒T淋巴细胞为主的杀瘤细胞，称其为TAK细胞。它是异质性细胞群，其细胞表型以CD8＋细胞为主，具有活化的淋巴母细胞的形态学特征，常形成集落，并表达活化的淋巴细胞的表面标记，如IL－2受体。

The soluble tumor antigen (STA) extracted from the specimens of the laryngeal squamous cell carcinoma could greatly proliferate the peripheral blood mononuclear cells (PBMC) compared to the recombinant interleukin-2 (rIL-2) alone or anti-CD, mAbalone or their different combinations (P<0. 01). We called the STA activated killer cells as TAK (tumor antigen activated killer) cells. This paper reported the preparationof STA and the morphology and the phenotype of TAK cells. The results indicated that TAK cells were heterogenous cell populations consisting predominantly of CD, positive T lymphocytes (81. 65%). The morphology of TAK cells was similar to that of the lymphoblasts which could keep a good state of proliferation and form colonies. TAK cells expressed IL-2 receptor α-chain characteristic for the activated T lymphocytes.