头颈部肿瘤患者自然杀伤细胞活性及计数分析

Analysis of activity and quantity of natural killer cell on patients with head and neck carcinoma

本文对25例头颈部肿瘤患者的外周血NK细胞杀伤活性及NK细胞计数进行测定，其中有15例患者手术治疗切除肿瘤，于术后三周复查NK活性及NK计数，并与正常人组对照。实验结果显示：头颈部肿瘤患者NK活性及NK计数均低于正常人，两组比较均有极显著性差异（P＜0．01）；术后患者的NK活性比术前有明显提高，配对t检验P＜0．01，NK计数比术前也有所提高，配对t检验P＜0．05，有显著性差异；NK活性的水平与肿瘤的分期有相关性，晚期（T4期）癌症患者NK活性明显低于早期（T1、T2）患者，可以看出NK活性随着肿瘤的发展而降低，肿瘤对宿主有免疫抑制作用；NK活性与NK数量之间无显著的相关性，二者之间的相关系数r＝0．344。

The activity and quantity of natural killer cell (NK) were measured in the peripheral blood of 25 patients with head and neck carcinoma. Fiveteen patients had their tumors resected. Three weeks after operation, the activity and quantity of NK cells were examinaed again, and compared with that in normal control. The results showed that: (1) The activity and quantity of NK cells in patients were lower than that in the control (P<0.01) . (2) The activity and quantity of NK cells increased in operated patients compared to those in patients before operation (P<0. 05). So tumor resection was helpful to increase the activity and quantity of NK cells in the patient and played an important role in removing the suppression of tumor to the body. (3) There was a relationship between the activity of NK cells and the tumor stages, the activity of NK cells in patients with advanced stage cancer were lower than that of early stage. It suggested that the activity of NK cells were reduced with the growth of tumor. (4) There didn't exist a marked relationship between the activity and quantity of NK cells (r=0. 344, P>0. 05 ). The results revealed that examination of the activity of NK cells was more improtant than measuring the quantity of NK cells in the patients.