栀子总苷对小剂量阿司匹林致胃黏膜损伤大鼠的保护作用

Protective effect of total glycosides of Zhizi on gastric mucosal lesion induced by low dose aspirin

目的研究栀子总苷(TGZ)对小剂量阿司匹林所致胃黏膜损伤的保护作用。方法采用TGZ(140,70,35 mg.kg-1)与小剂量阿司匹林(5.0 mg.kg-1)连续ig给药14 d,观察栀子总苷140、70、35 mg.kg-13个剂量组和对照组的胃黏膜损伤指数,并同时检测血清一氧化氮(NO)含量和一氧化氮合酶(NOS)的活性,以及胃组织中细胞间黏附分子(ICAM-1)的表达。结果TGZ用药组的胃黏膜损伤指数较模型对照组显著降低;与模型组比较,NO含量与NOS活性增加。免疫组化结果显示ICAM-1在模型组表达明显高于正常对照组,TGZ预防给药组可减少ICAM-1在胃组织中的表达。结论栀子总苷对小剂量阿司匹林所致胃黏膜损伤有明显的保护作用。

Aim To study the protective effect of total glycosides of Zhizi （TGZ） on gastric mucosal lesion induced by low dose aspirin in rats. Methods TGZ （ 140,70,35 mg· kg^-1 ） and low dose aspirin（5.0 mg· kg^-1 ,ig） were administered for a continuous 14 d. The lesion index of rats in TGZ （ 140,70,35 mg· kg^-1 ） and control groups were measured. The content of nitric oxide （NO） and nitric oxide synthase （NOS） level in blood and expression of intercellular adhesion molecule （ ICAM-1 ） were determined as well. Results Gastric mucosal lesion index of all TGZ dosage groups was obviously reduced compared with model group. Compared with model group,TGZ （ 140,70,35 mg · kg^- 1, ig, 14 d）could raise NO content and NOS activity. Immunological histology examination showed that ICAM-1 expression increased evidently in model group,and TGZ could degrade the expression of ICAM-1 in gastric tissue. Conclusion TGZ can inhibit gastric mucosal lesion induced by low dose aspirin.