禽冠状病毒M蛋白B细胞抗原表位的预测

Prediction of the epitope of B cell in avian coronavirus M protein

目的预测禽冠状病毒（IBV）M蛋白B细胞抗原表位。方法采用SOPMA软件预测IBV M蛋白的二级结构,采用SOSUI软件预测M蛋白的跨膜区,应用Hopp＆Woods方案、Janin方案、Zimmerman方案预测IBV M蛋白B细胞表位抗原。结果IBV M蛋白二级结构主要包括α-螺旋和无规卷曲,含量分别为32.89%、31.11%;M蛋白具有3个跨膜区,位于18～40、48～70、77～99位氨基酸;B细胞识别的表位可能位于第186-196位氨基酸区域内或附近,预测的表位均含有β-转角和无规卷曲结构。结论IBVM蛋白的二级结构和抗原表位比较稳定,可利用地方毒株的M蛋白制备基因工程疫苗和单克隆抗体,应用于该病的诊断和防治。

Objective To predict the epitope of B cell in avian coronavirus M protein. Methods The secondary structure of IBV M protein was predicted by SOPMA software and the transmembrane domain was predicted by SOSUI software. The epitope of B cell in IBV M protein was predicted with Hopp ＆ Woods,Janin and Zimmerman programs. Results The secondary structure of avian coronavirus M protein included α-helix and random coil, and the contents were 32.89 percent and 31.11 percent respectively. M protein had three transmembrane domains, which lied in the region of eighteen to fourty, forty-eight to seventy and seventy-seven to ninty-nine amino acids. The epitope of B cell may lie in the region of 186-196 amino acids or in the vicinity, which all contain β-turn and random coil structure. Conclusion The secondary structure and epitope of avian coronavirus M protein were stabilize, So this can be used for the diagnosis and prevention of the disease, through the preparation of genetically engineered vaccines and monoclonal antibodies by the local strain of M protein.