龈沟液中基质金属蛋白酶-9及基质金属蛋白酶组织抑制剂-1与牙周炎的关系

Correlation of matrix metalloproteinase-9 and tissue inhibitors of metalloproteinase-1 in gingival crevicular fluid with periodontal inflammation

目的观察慢性牙周炎患者在牙周基础治疗前后龈沟液中基质金属蛋白酶-9（MMP-9）、基质金属蛋白酶组织抑制剂-1（TIMP-1）水平的变化。方法采用滤纸条牙周袋内取样法提取15例慢性牙周炎患者在牙周基础治疗前以及基础治疗结束后1个月的龈沟液样本，同时提取15例牙周健康者的龈沟液样本，采用免疫印迹法检测MMP-9与TIMP-1水平。结果慢性牙周炎患者治疗后龈沟液中MMP-9水平[（1．2±0．9）AU]较治疗前[（2．2±1．4）AU]显著下降，TIMP-1水平[（3．7±2．2）AU]较治疗前[（1.3±1．2）AU]显著升高，P〈0．05；MMP-9、TIMP-1水平分别与牙周病临床参数呈显著正相关、显著负相关。结论MMP-9、TIMP-1水平反映了牙周组织的破坏程度，可作为评价疗效的客观指标。

Objective To examine the changes of matrix metalloproteinase-9 （MMP-9） and tissue inhibitors of metalloproteinase-1 （TIMP-1 ） in gingival crevicular fluid after conventional mechanical therapy of adult patients with chronic periodontitis. Methods Gingival crevicular fluid samples from 15 patients and 15 healthy subjects were taken with filter paper strips by intra-pocket method initially, respectively, 1 month after scaling and root planning. MMP-9,TIMP-1 levels were measured by immunoblotting. Results MMP-9 levels in patients were reduced significantly after treatment [ （2.2±1.4）AU vs （ 1.2 ± 0.9）AU ]. The clinical parameters were positive correlation with MMP-9 level. TIMP-1 levels in patients were increased significantly after treatment[ （ 1.3 ± 1.2 ）AU vs （3.7 ± 2.2）AU ]. The clinical parameters were negative con＇e-lation with TIMP-1 level. Conclusion MMP-9, TIMP-1 levels can act as objective parameters to evaluate the effect of periodontal therapy.