摘要
Ultra performance liquid chromatography(UPLC) coupled with orthogonal acceleration time-of-flight(oaTOF) mass spectrometry is becoming an effective and high-throughput analytical technique in metabonomics research.In this paper,an UPLC-oaTOF-MS system was employed to investigate the serum profiles of 40 diabete mellitus(DM)/diabetic nephropathy(DN) patients and 25 healthy volunteers in an effort to find potential biomarkers.The UPLC system produced information-rich chromatograms with typical measured peak widths of 4 s,and generated peak capacities of 225 in 15 min.After the UPLC-oaTOF-MS analysis,principal component analysis(PCA) was used for group differentiation and marker selection.In the projection of positive mode,DM/DN patients and the controls were obviously divided into three groups.Furthermore,two potential markers were identified as phytosphingosine and sphinganine,respectively.Phytosphingosine and sphinganine with decreased concentrations in the patients,were closely related to the sphingolipid metabolism.
Ultra performance liquid chromatography （UPLC） coupled with orthogonal acceleration time-of- flight（oaTOF） mass spectrometry is becoming an effective and high-throughput analytical technique in metabonomics research. In this paper, an UPLC-oaTOF-MS system was employed to investigate the serum profiles of 40 diabete mellitus（DM）/diabetic nephropathy （DN） patients and 25 healthy volunteers in an effort to find potential biomarkers. The UPLC system produced information-rich chromatograms with typical measured peak widths of 4 s, and generated peak capacities of 225 in 15 min. After the UPLC-oaTOF-MS analysis, principal component analysis （PCA） was used for group differentiation and marker selection. In the projection of positive mode, DM/DN patients and the controls were obviously divided into three groups. Furthermore, two potential markers were identified as phytosphingosine and sphinganine, respectively. Phytosphingosine and sphinganine with decreased concentrations in the patients, were closely related to the sphingolipid metabolism.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2008年第11期2171-2173,共3页
Chemical Journal of Chinese Universities
基金
中国博士后科学基金(批准号:20080430108)
国家自然科学基金(批准号:20775063)资助
关键词
糖尿病
糖尿病肾病
代谢组学
超高效液相色谱
飞行时间质谱
Diabete mellitus
Diabetic nephropathy
Metabonomics
Ultra performance liquid chromatography
Time-of-flight mass spectrometer
