海洋放线菌Streptomyces sp. 124092中的细胞毒活性成分

Cytotoxic Constituents from Marine Actinomycete Streptomyces sp. 124092

对海洋放线菌Streptomyces sp.发酵液的提取物进行柱层析分离,得到5个化合物.经MS,NMR,^1H-^1HCOSY,HSQC和HMBC等数据鉴定,其结构分别为3-氨基-丙酸对甲苯酯（1）、6-Amino-3-（4-hydroxy-benzyl）-1,4-diazonane-2,5-dione（2）、正丁基-α-D-吡喃甘露糖苷（3）、大豆苷元（4）和1H-3-吲哚甲酸（5）.其中化合物1和2为新化合物,细胞毒活性测试结果表明,化合物1～4对人肝癌细胞（SMMC-7721）具有不同程度的生长抑制活性.

To study the cytotoxic constituents from marine actinomycete Streptomyces sp. （124092） of rhizosphere soil of mangrove plant Heritiera littoralis, five compounds were isolated by column chromatography from the fermentation broth of marine actinomycete Streptomyces sp. （124092）. Their structures were elucidated as p -tolyl 3 -aminopropanoate （ 1 ）, 6 -amino -3 - （ 4 -hydroxybenzyl ） - 1,4 -diazonane -2,5-dione （ 2 ）, n-butyl manno- side（3）, daidzein（4）, and indole-β-carboxylic acid（5）, by MS, NMR, ^1H-^1H COSY, HMQC, and HMBC spectra. Among them, compounds 1 and 2 are new compounds. Cytotoxic bioassay results show that com- pounds 1-4 possess inhibitory effects on human hepatoma SMMC-7721 cell line in vitro.