HPLC法测定清脑降压胶囊中黄芩苷的含量

Quantitative determination of baicalin in Qingnaojiangya Capsules by HPLC

目的建立以高效液相色谱法测定清脑降压胶囊中黄芩苷含量的方法。方法样品前处理采用超声提取方法；色谱柱为Agilent zorbax Eclipse XDB-C18（4．6mm×250mm，5μm），流动相为甲醇-0．2％磷酸水溶液（42：58）；检测波长278nm；流速为0．8mL／min。结果黄芩苷在0．366—1．832μg范围内呈良好的线性关系；相关系数分别0．9991；黄芩苷的平均加样回收率为99．84％（RSD为0．969％）。结论该方法灵敏，简便，结果准确，适用于清脑降压胶囊中黄芩苷含量测定。

Objective To establish a method for quantitative determination of baicalin in Qingnaojiangya Capsules by HPLC. Methods The ultrasonic extracting method was applied in sample pre-treatment. The HPLC procedure was performed on the chromatographic column of Agilent zorbax Eclipse XDB-C18 （4.6 mm × 250 mm, 5 μm） , the mobile phase was methano1-0. 2% of phosphoric acid water solution （42： 58）. The detection wavelength was 278 nm and flow velocity was 0.8 mL/min. Results Baicalin showed a good linear relationship at range of 0.366 - 1. 832 μg with correlation coefficient of 0.999 1. The average recovery of baicalin samples was 99.84% （ sensitive, simple and reliable, which was can be used Qingnaojiangya Capsules. RSD =0.969% ）. Conclusion The method was for the quantitative determination of baicalin inQingnaojiangya Capsules.