健胃愈疡片干预下慢性胃炎伴HP感染胃黏膜PAF的表达

Expressions of platelet activating factors of gastric mucosa intervened by Jianweiyuyang Pills in chronic helicobacter-pylori-related gastritis

目的观察健胃愈疡片对幽门螺杆菌(HP)感染慢性胃炎患者黏膜血小板活化因子(PAF)表达的变化,并分析其可能的作用机制。方法选取经胃镜证实的HP感染慢性胃炎患者80例,随机分为2组,治疗组42例给予健胃愈疡片治疗,对照组38例给予西药三联药物治疗。应用胃镜下活检胃黏膜组织快速尿激酶和Warthin-Starry染色法检测幽门螺杆菌,并同时检测血小板活化因子表达的变化和氨基己糖及磷脂含量。第4周复查胃镜。结果治疗组总有效率与对照组接近,健胃愈疡片治疗前磷脂和氨基己糖的含量显著高于治疗后(P<0.01)。健胃愈疡片组氨基己糖、磷脂含量增高,与对照组比差异有显著性(P<0.01)。健胃愈疡片可下调PAF的表达,而对照组对胃黏膜PAF的表达影响不大。结论健胃愈疡片抗胃炎的作用机理可能有以下几点:杀灭HP,下调PAF的表达,影响胃黏膜氨基己糖及磷脂含量,影响胃粘膜疏水性,加强黏液凝胶层稳定性,从而防止胃炎的产生和复发。

Objective To observe the effect of Jianweiyuyang Pills on the expression changes of platelet activating factors （PAF） of gastric mucosa in the patients with chronic helicobacter-pylori-related gastritis, and study the potential mechanism. Methods The patients with chronic helicobaeter-pylorirelated gastritis （ n = 80, finally diagnosed two groups, including the treatment group （ by gastroscope） were chosen, and then randomly divided into n = 42） treated with Jianweiyuyang Pills and the control group （n = 38） treated with three combined Western medicates. The pykno-urokinase in the gastric mucosa was detected by using gastroscopic biopsy, the helicobacter pylori was tested by Warthin-Starry stain method, and the expression changes of PAF and content of aninohexose and phosphatide were determined at the same time. On the forth week the patients were given reexamination with gastroscopy. Results The total effective rate of the treatment group was similar to that of the control group. The contents of aninohexose and phosphatide were significantly higher after the treatment than before （P 〈 0.01 ）. In the treatment group the contents of aninohexose and phosphatide increased with a significant difference compared with those in the control group （ P 〈 0.01 ）. The expression of PAF was down-regulated by Jianweiyuyang Pillsin the treatment group but had no changes in the control group. Conclusion Jianweiyuyang Pills for anti-gastritis may be related to killing helicobacter pylori, The mechanism of down-regulating PAF expression, influencing the contents of aninohexose and phosphatide, effecting on the hydrophobicity of gastric mucosa, strengthening the stability of gastric mucus gel layer, and preventing gastritis from recurrence.