应用PCR法检测庚型肝炎病毒感染

Detection of HGV Infection by RT Nested PCR Technique

应用逆转录套式ＰＣＲ法检测了４８例丙型肝炎、２６例乙型肝炎及１２例甲乙丙丁戊型肝炎病毒感染标志均阴性的肝炎患者血清中的庚型肝炎病毒（ＨＧＶ）ＲＮＡ，结果表明我国肝炎患者中存在ＨＧＶ感染；ＨＧＶ可单独感染或与其它肝炎病毒混合感染，丙型肝炎患者中，ＨＧＶＲＮＡ的检出率为１０．４％，乙型肝炎患者中的检出率为３．８％，而在甲乙丙丁戊型肝炎标志均阴性的肝炎患者中的检出率为３３．３％，ＨＧＶ与其他肝炎病毒的混合感染可加重肝脏的损害。

RT nested PCR method were used to detect HGV RNA in sera of 48 cases of hepatitis C,26 cases of hepatitis B and 12 cases of hepatitis with unidentified pathogen.The results showed that there was HGV infection in patients with liver diseases in our country.HGV can infects hepatocytes alone or take a co-infection with HCV or HBV.The positive rates of HGV RNA in the groups of hepatitis C,Hepatitis B and hepatitis with unidentified pathogen were 10.4%,3.8% and 33.3% respectively.The co-infection of HGV with HCV or HBV may aggravate liver damage.HGV infection is one of the pathogens of non A～E hepatitis in our country.