β-CD、DBS协同增敏美他环素的荧光光谱研究及分析应用

Studies and Application of Fluorescence of Metacycline Enhanced by Synergetic Effect of β-Cyclodextrin and DBS

用荧光光度法研究了阴离子表面活性剂十二烷基苯磺酸钠(DBS)-β-环糊精(β-CD)体系对美他环素的协同荧光增敏作用。在β-CD-DBS体系中美他环素的荧光强度显著增强,由此建立了测定美他环素的新方法。结果表明:美他环素与DBS、β-CD生成摩尔比为1∶1∶1的三元包合物,其表观结合常数为1 294 L2.mol-2。美他环素浓度在1.7×10-7～8.8×10-5mol.L-1范围内与体系荧光强度呈线性关系,相关系数r为0.996 6,检测限为7.4×10-9mol.L-1。方法用于测定药物制剂中美他环素的含量,回收率在95%～106%之间,结果令人满意。

The fluorescence of metacycline enhanced by the synergetic effect of β-cyclodextrin with anion surfactant sodium dodecylbenzene sulfonate（DBS） was studied.It was found that the fluorescence intensity of metacycline was greatly enhanced in the system of β-CD-DBS.Based on this a new sensitive method for the determination of metacycline was established.The experiments indicated that MTCβ-CD-DBS can form 1∶1∶1 ternary inclusion complex with apparent stability constant of 1 294 L2·mol-2,respectively.The fluorescence intensity was linearly related to the metacycline concentration in the range of 1.7×10-7 to 8.8×10-5 mol·L-1 with a correlation coefficient of 0.996 6.The detection limit was 7.4×10-9 mol·L-1 this method was satisfactorily applied to the determination of metacycline in pharmaceutical preparations.The recovery was within the range of 95% to 106%.