大肠杆菌生产重组人bFGF的发酵条件

A Study on Fermentation Conditions of Recombinant Human bFGF in Eschierichia Coli

对含有具氨苄抗性、Ｌａｃ启动子和编码人碱性成纤维细胞生长因子（ｈ－ｂＦＧＦ）基因质粒的大肠杆菌的发酵条件进行了研究．为了获得ｈ－ｂＦＧＦ高效表达的最佳条件，进行了摇瓶试验．在ＩＰＴＧ诱导作用下，ｈ－ｂＦＧＦ可以分泌到胞浆中．采用连续流加葡萄糖的高密度分批发酵工艺，经１１ｈ发酵可以获得菌体光密度值（ＯＤ值）达３８，产量达２００ｍｇ／Ｌ的ｈ－ｂＦＧＦ．经纯化后得到的高纯度ｈ－ｂＦＧＦ其理化及生物学特性。

Fermentation studies were preformed on an Eschericha coli culture that carries a recombinant plasmid of an amolicillin resistent gene,lac promotor,and ecoding human basic fibroblast growth factor(h bFGF).The objective was to achieve optimum fermentation conditions while maintaining the specific expression level of recombinant h bFGF observed in shakef lasks.Upon induction with isoprophylthio β D galactoside(IPTG) the culture ssecreted the protein to the plasm.Fed batch ferentation with stepwise addition of glucose during the active growth phase of the organism resulted in the production of 200 mg/L h bFGF in 11hours and a cell optical density(OD) of 38.The h bFGF was purified to homogeneity from the culture and was found to be chemically and biologically identical to the native protein by western bloting. N terminal amino acid sequencing and biological assay.