摘要
本文报道了应用各种基团修饰剂对Bsp63I及其底物进行化学修饰的研究．结果表明：锍基及赖氨酸残基可能是该酶活性中心的必需基团；酶识别序列中的d（Gc）碱基在催化过程中具重要作用．
This paper reports on a study of the chemical modification of Bsp63 Ⅰ and itssubstrate by using group modification reagents. The results show that the sulfhydryl groupand the lysine residue are possibly the necessary group in the active centreof Bsp63 Ⅰ. Thebase sequence of d(Gc),which is in the recognition sequence of the Bsp63 Ⅰ,plays an important role in the catalysis of Bsp63 Ⅰ.
出处
《湖南教育学院学报》
1997年第2期91-94,共4页
Journal of Hunan Educational Institute
关键词
化学修饰
识别序列
Bsp63I
限制酶
工具酶
restriction endonuclease
chemical modification
necessary group
recognition sequence
