RT-PCR检测SALL4基因在白血病中的表达

Detection of SALL4 mRNA expression in leukemia cells by RT-PCR

目的:检测SALL4基因在白血病细胞中的表达情况。方法:运用RT-PCR技术对35例白血患者及10例正常对照的单个核细胞进行SALL4mRNA表达测定。结果:急性非淋巴细胞白血病(ANLL)患者中,SALL4mRNA表达率为83.3(15/18);慢性髓细胞白血病(CML)患者SALL4mRNA表达率为100(7/7);B-ALL患者中,SALL4mRNA表达率为100(6/6),T-ALL的SALL4mRNA表达率为0(0/4)。对照组10例的SALL4mRNA表达为阴性。ANLL、CML、B-ALL之间SALL4mRNA的表达率比较差异无统计学意义(P>0.05),前3组与T-ALL、对照组间SALL4mRNA的表达差异有统计学意义(P<0.01)。BCR-ABL融合基因与SALL4在ALL、CML组中的表达呈正相关性。结论:ANLL、CML、B-ALL的单个核细胞SALL4mRNA表达阳性,提示SALL4基因可能与白血病的发生相关;B-ALL与T-ALL间的SALL4基因表达差异有统计学意义,提示2者的发病机制可能不同。

Objective：To detect SALL4 mRNA expression in leukemia cells. Method：RT-PCR method was used for detecting SALL4 mRNA expression in the mononuclear cells of 35 patients with leukemia and 10 normal controis. Result：The positive rates of SALL4 mRNA expression were 83.3% （15 of 18） in patients with ANNL, 100% in patients （7/7） with CML,100% （6 of 6） in patients with B-ALL and 0 （0/4） in patients with T-ALL. Expression of SALL4 mRNA was negative in 10 normal controls. In the positive rates of SALL4 mRNA expression among ANLL, CML and B-ALL, the differences were not statistically significant （ P 〉0.05）. In the expression rates of the previous three groups with T-ALL and normal controls respectively, the differences were statistically significant （ P 〈0.01）. We found there was obvious relationship between the expression of BCR-ABL gene and SALL4 mRNA in the cases with CML or ALL by association analysis. Conclusion： Expression of SALL4 mRNA was positive in the mononuclear cells of the patients with ANLL, CML and B-ALL. The results suggested that SALL4 mRNA may be related to the genesis of leukemia. The significant difference between expression rates in cases with BALL and T-ALL indicates that there is variant pathogenesis between them.