基于FePz(dtn)_4和纳米金固定辣根过氧化物酶生物传感器的研究

A Biosensor Based on Immobilizing Horseradish Peroxidase on FePz(dtn)_4 and Au Colloid as Matrixes

在玻碳电极上电沉积四(1,4-二噻英)四氮杂卟啉铁(FePz(dtn)4),将其作为电子媒介体与纳米金结合,利用纳米金的比表面积大,吸附能力强等优点固定辣根过氧化物酶,最后用聚乙烯缩丁醛包埋修饰好的电极,制备了过氧化氢传感器.采用循环伏安法考察了传感器的电化学特性,以-0.25V为工作电位,在pH=6.5,温度为25℃的最佳条件下,其峰电流值与H2O2浓度在3.5×10-6～7.35×10-3mol/L成良好的线性关系,检测下限为1.0×10-6mol/L(S/N=3).该传感器有非常好的稳定性,响应较快、灵敏度较高,且具有良好的选择性.

A novel application of Iron （ Ⅱ ）-Tetra （5,6-dihydro- 1,4-dithijn） porphyrazine, i.e. FePz （dtn） 4, on the Hydrogen Peroxide Biosensor. FePz（dtn）4 was fixed on the glass carbon electrode by electrodeposition method. Then colloidal Au was used to adsorb horseradish peroxidase, and polyethylene butyral was used to embed modified electrode. Cyclic voltammetry was employed to demonstrate the feasibility of electron transfer between peroxidase and electrode. Linear calibration for H2O2 was obtained in the range from 3.5 ×10^-6to 7.35 ×10^-3 mol/L, the correlation coefficient being 0. 999 4 and the limit being 1.0 ×10^-6 mol/L at a signal-to-noise ratio of 3. The sensor had good sensitivity, selectivity and stbility.