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水飞蓟宾对转化生长因子β1诱导的人肾小管上皮细胞中结缔组织生长因子及Ⅲ型胶原表达的影响 被引量:2

The effect of silibinin on the expression of connective tissue growth factor(CTGF)and the type Ⅲ collagen in HK-2 cells induced by TGF-β_1
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摘要 目的:了解水飞蓟宾(silibinin)对转化生长因子(TGF)-β_1诱导的人近曲肾小管上皮细胞(HK-2)结缔组织生长因子(CTGF)表达及Ⅲ型胶原分泌的影响。方法:将体外培养的HK-2细胞分为3组:①对照组;②TGF-β_1组(TGF-β_1的终浓度为5 ng/ml);③TGF-pβ_1+水飞蓟宾组(TGF-β_1的终浓度为5 ng/ml,水飞蓟宾终浓度分别为5、10、20μg/m1)。48 h后,采用逆转录聚合酶链反应(RT-PCR)检测细胞内CTGFmRNA的水平,ELISA方法检测培养上清中胶原Ⅲ及CTGF的浓度。结果:与对照组比较,TGF-β_1刺激使HK-2细胞CTGF基因表达和蛋白分泌及Ⅲ型胶原分泌均显著增加(P<0.01)。与TGF-β_1组比较,加用5~20μg/ml水飞蓟宾可以抑制TGF-β_1刺激所诱导的CTGF基因表达和蛋白分泌及Ⅲ型胶原分泌(P<0.01),以20μg/ml水飞蓟宾效果最显著。结论:水飞蓟宾能部分抑制TGF-β_1诱导CTGF的基因和蛋白的表达及Ⅲ型胶原分泌,提示水飞蓟宾可能通过阻抑CTGF表达及减少细胞外基质的积聚,具有潜在的抗肾纤维化作用。 Objective:To investigate the effect of silibinin on the expression of connective tissue growth factor (CTGF) and the secretion of type Ⅲ collagen in HK-2 cells induced by TGF-β1. Methods:In vitro,HK-2 cells were divided into three groups: (1)ontrol group; (2)5 ng/ml TGF-β1, group; (3)5 ng/ml TGF-β1+silibinin with different concentrations group, 5μg/ml, 10μg/ml and 20μg/ml, respectively. 48 h later,the expression of CTGF mRNA in cells were detected by reverse transcription-polymerase chain reaction(RT- PCR) ;the concentrations of CTGF and collagen Ⅲ secreted into the culture supernatant was determined by ELISA. Results:Compared with control group,the expression of CTGF mRNA and the concentration of the secreted collagen Ⅲ and CTGF were all increased markedly in the group of TGF-β1 treatment (P 〈 0.01). Compared with TGF-β1 group,the different concentrations of (5μg/ml, 10μg/ml and 20 μg/ml) silibinin could also effectively suppress the expressions of CTGF mRNA and the concentration of the secreted col lagen Ⅲ and CTGF (P 〈 0.01 ) in a dose-dependent manner. And the 20μg/ml silibinin group had the most prominent effect. Conclusion: Silibinin could suppress the expressions of collagen Ⅲ and CTGF induced by TGF-β1. It is suggested that silibinin might exert an inhibitive effect on renal fibrosis,which may be related with its role in reducing the expression of CTGF and preventing accumulation of ECM.
出处 《南京医科大学学报(自然科学版)》 CAS CSCD 北大核心 2008年第11期1381-1384,共4页 Journal of Nanjing Medical University(Natural Sciences)
基金 南京医科大学科技发展基金面上项目(06NMUM030)
关键词 水飞蓟宾 转化生长因子-Β1 结缔组织生长因子 Ⅲ型胶原 silibinin TGF-β1 connective tissue growth factor type Ⅲ collagen
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参考文献15

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共引文献1

同被引文献41

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