生脉散正交实验抗心律失常作用研究

Study on the Effect of Shengmaisan on Arrhythmia through Othorgonal Experiment

目的:本实验通过研究由氯化钡引起小鼠的室性心律失常,筛选出生脉散的抗心律失常的最佳组方。方法:灌胃给药不同组方生脉散水煎液10天,麻醉小鼠,腹腔注射0.3%氯化钡,建立室性心律失常模型。结果:经过正交实验所得最佳配比为人参18g,麦冬18g,五味子6g。所得最佳配比组与经典配比进行实验比较,经典组(人参9g,麦冬9g,五味子6g)对心律失常的治疗作用最显著,为最佳组方。

Objective： To study experimental arrhythmia in mice caused by Baritum Chloride screening the best composing prescription of Shengmaisan used for anti- arrhythmia. Methods： Intragastric gavage with different shengnai powders water- decocted liquid for tan days. injected mice, leave them at the stage of anestjesia, injected intraperitoneally the mouse with 0.3% Barium chloride. Making the arrhythmia mice animal model with Barium chloride. Results： Compare the result from orthogonal test, the optimal prescription of Shengnaisan is Radix Ginseng （18g）, Radix Ophiopogonis （18g）, Fructus Schisandrae （6g） . Compare the best camposing prescriptions.with the classical matCh of Shengnaisan , the classical match of Shengmaisan （Radix Ginseng 9g, Radix Ophiopogonis 9g, Fructus Schisandrae 6g） make the most avident role , so this is the best composing prescriptions.