冻干辐照猪硬脑膜胶原酶酶解时间与生物力学的测定

Determination of freeze-dried and irradiated porker dural resistant ability to collagenase digestion and biomechanics

背景:理想的硬脑膜替代材料要求其自身具有一定的韧性与弹性。目的:通过对经冻干辐照处理后猪硬脑膜胶原酶Ⅰ型酶解时间、最大负荷及抗张强度进行测定,验证经冻干、辐照处理过的猪硬脑膜是否符合硬脑膜移植对生物力学的要求。设计、时间及地点:对比分析实验,于2007-07/2008-06在南昌大学烧伤研究所、南昌大学-新三思公司联合力学实验室、江西中医学院中药固体制剂国家工程研究中心与江西天兆科技发展公司完成。材料:选用健康活体猪6只,无菌条件下取新鲜猪硬脑膜。方法:猪硬脑膜经过冲洗、预冻、冻干、60Coγ射线照射等处理,按照处理方式分成新鲜猪硬脑膜组、冻干猪硬脑膜组、辐照猪硬脑膜组及冻干+辐照猪硬脑膜组。主要观察指标:取5mm各组猪硬脑膜圆片用胶原酶Ⅰ型酶解计算其完全酶解时间;用EMT彩屏电子拉力试验机分别测出各组替代材料的最大负荷、抗张强度。结果:新鲜猪硬脑膜组、冻干猪硬脑膜组、辐照猪硬脑膜组及冻干+辐照猪硬脑膜组硬脑膜胶原酶酶解时间分别为(8.3±2.5),(7.6±1.8),(23.6±5.7)与(21.1±5.3)min,最大负荷和抗张强度分别为(79.93±4.36),(70.50±5.97),(96.97±4.84)N和(93.59±4.61),(7.98±0.44),(7.05±0.60),(9.70±0.48)和(9.40±0.46)N/mm,与新鲜猪硬脑膜组和冻干猪硬脑膜组比较,辐照猪硬脑膜组和冻干+辐照猪硬脑膜组时间明显延长,差异有显著性意义(P<0.05),但辐照猪硬脑膜组及冻干+辐照猪硬脑膜组结果无明显差别(P>0.05)。结论:经Coγ射线照射后的硬脑膜抗酶解能力、生物力学特性增强;辐照与冻干+辐照猪硬脑膜均能满足硬脑膜移植对其生60物力学方面的要求。

BACKGROUND： Ideal dural substitute materials requires its good biomechanics properties, tenacity and elasticity. OBJECTIVE： To determine freeze-dried and irradiated porker dura＇s resistant ability, including type I collagenase digestion, the biggest load and tensile strength, to assess the porker＇s dura irradiated and freeze-dried whether or not can satisfy the biomechanics requirment in dura draft. DESIGN, TIME AND SETTING： This controlled analysis experiment was performed at the Bum Institute, Nanchang University, Association Mechanics Laboratory, Nanchang University-New Sans Company, Chinese Crude Drug Solid Preparation Nation Project Research Center of Jiangxi Traditional Chinese Medical College and Jiangxi Tianzhao Technology Development Company from July 2007 to June 2008. MATERIALS： Six healthy living pigs was used to sterilely obtain fresh dura mater. METHODS： After washing, pre-frozen, freeze-dried and radiation by 60Co γ -ray, we prepared fresh, freeze-dried, irradiated and freeze-dried plus irradiated pig dura. MAIN OUTCOME MEASURES： 5-mm wafer of four group dura were dealt with type Ⅰ collagenase meanwhile, digested time was recorded. EMT color screen electronic testing machine was used to measure the biggest load and tensile strength of the porker dura in each group. RESULTS： Time of the dura resistance to collagenase digestion were respectively （8.3 ± 2.5）, （7.6 ± 1.8）, （23.6 ± 5.7） and （21.1 ±5.3） minutes, and the largest load and tensile strength were （79.93 ± 4.36）, （70.50± 5.97）, （96.97 ± 4.84） N and （93.59 ± 4.61）, （7.98±0.44）, （7.05±0.60）, （9.70±0.48） and （9.40±0.46） N/mm in the fresh, freeze-dried, irradiated, and freeze-dried plus irradiated pig dura groups. Compared with the fresh and freeze-dried pig dura groups, the time was longer in the irradiated and freeze-dried plus irradiated pig dura groups （P 〈 0.05）. However, there was no significant difference between the irradiated and freeze-dried plus irradiated pig dura groups （P 〉 0.05）. CONCLUSION： Dural substitute materials irradiated by 60Co γ-ray enhanced the ability against enzymatic hydrolysis and biomechanics. Irradiated and freeze-dried plus irradiated dural substitution can meet the requirements of the biomechanics in dura mater transplantation.