生物新材料细菌纤维素对深Ⅱ度烧伤大鼠皮肤的治疗作用

Effect of nanometer biomaterial bacterial cellulose on the deep second-degree burn in rats

背景:研究表明细菌纤维素有利于皮肤组织生长和限制感染,为制备用于烧伤患者和慢性皮肤溃烂患者的生物敷料及临时皮肤提供有利条件。目的:观察生物新材料细菌纤维素对深Ⅱ度烧伤大鼠皮肤的治疗作用。设计、时间及地点:随机对照动物实验,于2007-06/12在解放军第二军医大学动物实验中心完成。材料:木醋杆菌M12由山东轻工业学院酶技术研究室选育获得。清洁级成年健康SD大鼠30只。方法:菌株M12经液态30℃恒温静置培养6d发酵可产生不溶性的凝胶膜,用0.1mol/L的NaOH溶液,100℃煮沸20min,去除液膜中的菌体和残留培养基后,即得乳白色半透明的细菌纤维素膜。用自制烫伤仪于SD大鼠背部脊柱两侧各制造2.0cm×2.0cm大小的皮肤烫伤,随机选择一侧为治疗侧,表面敷以细菌纤维素膜,用1号手术缝线缝扎固定于周围皮肤上,另一侧为对照侧,不予包扎。主要观察指标:术后4,7,14,21,28d计算伤口愈合率。光镜下观察皮肤损害及愈合情况。结果:所有实验动物无死亡,均未出现创面感染,术后21d治疗侧伤口愈合率高于对照侧,差异有显著性意义(P<0.01);其他时间点两侧比较差异无显著性意义。组织学观察两侧病理损害和愈合程度在各个时间点无明显差异。结论:细菌纤维素膜在一定程度上具有促进烧伤创面愈合的作用,其作用效果还需要运用其他类型皮肤损伤的动物模型进一步验证。

BACKGROUND： Bacterial cellulose benefits the growth of skin tissue and limits the infection, it may provide advantageous conditions for the biomaterials dressing and temporary skin in the burnt patients and chronic ulcer patients. OBJECTIVE： To study the therapeutic effect of bacterial cellulose on the deep second-degree burn in rats. DESIGN, TIME AND SETTING： Randomized control animal experiments were performed in the Animal Experimental Center of the Second Military Medical University of Chinese PLA from June to December in 2007. MATERIALS： Acetobacter xylinum M12 was purchased from Enzyme Technique Department, Shandong Institute of Light Industry. Thirty healthy SD rats of cleaning grade were used. METHODS： M12produced non-soluble gel membrane after it was cultured 6 days at a constant temperature of 30℃, and then was boiled 20 minutes in 0.1 mol/L NaOH solution. Ivory semitransparent bacterial cellulose was obtained following the removals of thallus in liquid membrane and residual culture medium. Skin burn in a size of 2.0 cm×2.0 cm was made in bilateral sides of spinal cord on back with self-made wound meter. One side was treated with bacterial cellulose dressing and sutured, taking as experimental side. While the other side served as control side without dressing. MAIN OUTCOME MEASURES： At 4, 7, 14, 21 and 28 days after operation, the healing rate of skin burn in rats was calculated. The skin wound and healing were observed under light microscope. RESULTS： All animals were alive normally without the presence of wound infection. Compared with control side, the wound healing rate of treated side significantly improved after 21 days （P 〈 0.01）; there was no significant difference between the treated side and control side at other time points. The histological results indicated that the pathological lesion and healing had no significant differences between the two sides. CONCLUSION： Bacterial cellulose can promote the healing of skin burn wound at some degree, the therapeutical effect deserves more study on various skin wounds in animals.