肉毒碱复合高脂饲料喂养兔血脂水平的变化

Changes of serum lipid level in rabbits fed with carnitine combined with high-fat diet

背景:肉毒碱是脂肪酸进入线粒体内进行氧化代谢的惟一转运载体,其在机体内水平对血脂代谢具有重要的影响作用。目的:观察肉毒碱对高脂饲料喂养兔血脂水平变化的影响。设计、时间及地点:随机对照动物实验,于2006-10/2007-05在解放军南京军区南京总医院检验科及比较医学科完成。材料:普通级健康纯系雄性新西兰兔14只,体质量(1.98±0.22)kg,用于建立兔高脂血症模型。方法:14只雄性新西兰兔随机抽签法分为对照组及实验组,每组7只。对照组仅用高脂饲料喂养,实验组用高脂饲料喂养的同时每天补充肉毒碱200mg/kg体质量。分别于实验开始后第0,2,3,4周称体质量并抽取耳动脉血,分离血清后测定血中总胆固醇、三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇以及载脂蛋白B和血清游离肉毒碱水平。主要观察指标:不同时间点各组动物体质量的变化以及血中总胆固醇、三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇以及载脂蛋白B和血清游离肉毒碱水平。结果:14只新西兰兔全部进入结果分析。4周内,实验组和对照组体质量差异无显著性意义(P>0.05)。自第2周开始,实验组兔血清游离肉毒碱水平高于对照组(P<0.01);总胆固醇、载脂蛋白B水平明显低于对照组(P<0.05或P<0.01);3周后,实验组血清三酰甘油、低密度脂蛋白胆固醇水平也低于对照组(P<0.01);两组高密度脂蛋白胆固醇水平差异无显著性意义(P>0.05)。结论:补充服用肉毒碱能够抑制高脂饮食导致的血中总胆固醇、三酰甘油、低密度脂蛋白胆固醇以及载脂蛋白B水平升高。

BACKGROUND： Carnitine, which is the only carder for the fatty acid crossing into mitochondria for oxidize, has important effect on serum lipids metabolism. OBJECTIVE： To investigate the effect of carnitine supplement on the level changes of serum lipids in rabbits fed with high-fat diet. DESIGN, TIME AND SETTING： The randomized controlled animal experiment was performed at the Department of Clinical Laboratory, General Hospital of Nanjing Military Area Command of Chinese PLA from October 2006 to May 2007. MATERIALS： 14 male New Zealand rabbits, weighting （1.98±0.22） kg, were adopted to establish hyperlipidemia model. METHODS： 14 rabbits were randomly divided into experimental and control group, with 7 animals in each. Rabbits in the control group were fed with high-fat diet, and those in the experimental group were supplied with 200 mg/kg carnitine everyday combined with high-fat diet. The blood was collected from central artery and body weight was obtained at 0, 2, 3, 4 week after the experiment, then total cholesterol, triglyceride, high density lipoprotein cholesterol, low density lipoprotein cholesterol, apolipoprotein B and free carnitine levels in serum were measured. MAIN OUTCOME MEASURES： Changes of body wight, total cholesterol, triglyceride, high density lipoprotein cholesterol, low density lipoprotein cholesterol, apolipoprotein B and free carnitine levels in serum were measured at various time points in each group. RESULTS： All 14 rabbits were involved in the result analysis. Body weight of the rabbits between the experimental group and control group showed no difference in 4 weeks （P 〉 0.05）. From the second week, serum carnitine level in the experiment group was higher than that of the control group （P 〈 0.01）, total cholesterol and apolipoprotein B levels were significant lower than those in the control group （P 〈 0.05 or P 〈 0.01）. After the third week, the levels of triglyceride and low density lipoprotein cholesterol in the experiment group were lower than those in the control group （P 〈 0.01）. There was no significant difference of high density lipoprotein cholesterol level between the two groups （P 〉 0.05）. CONCLUSION： Carnitine supplement can inhibit the increasing of total cholesterol, triglyceride, low density lipoprotein cholesterol and apolipoprotein B levels in serum of the rabbits fed with a high-fat diet.