自体富血小板血浆促进兔上颌缝的牵张成骨(英文)

Autologous platelet-rich plasma promotes premaxillary suture distraction osteogenesis in rabbits

背景:富血小板血浆内含多种高浓度的生长因子,具有促进新骨生成,加速愈合的作用,对牵张成骨的作用尚无公识性结论。目的:观察自体富血小板血浆对兔上颌骨牵张成骨的影响。设计、时间及地点:随机对照动物实验,于2007-07/08在中山大学北校区何母实验楼生理实验室完成。材料:3～5月龄健康家兔16只,雌雄各半,体质量1.4～1.7kg,随机分为实验组和对照组,每组8只。自制上切牙带环、外置式前牵引面具、牵引橡皮圈。凝结剂由1000U牛凝血酶溶于1mL100g/L氯化钙制成。方法:分别在家兔左侧前颌缝的两侧置入钛钉(直径1.5mm),戴自制前牵引装置,试验组在牵张开始时,注射V(富血小板血浆)∶V(凝结剂)=9∶1混合的凝胶样物质至左侧前颌缝内。两组分别持续牵引1周和3周,每个时间点4只家兔。主要观察指标:牵引结束后测量骨缝两侧钛钉间距离的增加值以及组织学观察结果。结果:对照组和试验组家兔上颌均向前移位。同对照组相比,实验组骨缝标志钉间距离增加值较大,新骨生成与矿化较快,血管分布较多,牵张间隙中骨小梁较为粗壮和成熟。结论:富血小板血浆有助于骨组织再生,对兔上颌牵张成骨具有促进作用。

BACKGROUND： Platelet-rich plasma containing various high-concentration growth factors can promote new bone formation and accelerate bone healing. But its effects on distraction osteogenesis remain unclear. OBJECTIVE： To observe the effects of autologous platelet-rich plasma on premaxillary distraction osteogenesis in rabbits. DESIGN, TIME AND SETTING： A randomized controlled animal experiment was performed at the Laboratory of Physiology, North Campus, Sun Yat-sen University between July and August 2007. MATERIALS： Sixteen healthy rabbits, the same number for male and female, aged 3-5 months, weighing 1.4-1.7 kg, were randomly and evenly divided into an experimental group and a control group. Premaxillary incisor band, outside front distraction mask, and distraction rubber band were self-made. Coagulant was prepared by dissolving 1000 u bovine thrombin in 1 mL of 10% calcium chloride. METHODS： Titanium nails （1.5 mm in diameter） were separately inserted on two sides of left premaxillary suture in rabbits. A self-made distraction device was used. Gelatum-like substance [V platelet-rich plasma： V coagulant = 9：1] was injected into the left premaxillary suture of experimental group rabbits immediately prior to distraction. In each group, one-week sustained distraction was performed in 4 rabbits, and three-week sustained distraction was performed in another rabbits. MAIN OUTCOME MEASURES： The increase of nail-nail distance on the two sides and histological results following distraction osteogenesis. RESULLTS： Rabbit premaxillary bone moved anterior in the two groups. The experimental group showed greater increase of nail-nail distance, faster bone formation and mineralization, more blood vessels, and thicker and more mature bone trabecula in the distraction interspace in comparison with the control group. CONCLUSION： Platelet-rich plasma will help bone tissue regeneration and promote premaxillary distraction osteogenesis.