Soluble endoglin和sFlt-1在子痫前期患者血清中的表达

Expression of soluble endoglin and sFlt-1 in peripheral blood of pre-eclampsia

目的探讨可溶性CD105淋巴细胞抗原及可溶性血管内皮生长因子受体1(sFlt-1)在子痫前期患者血清中表达水平的变化及意义。方法采用双抗体夹心酶联免疫吸附(ELISA)法测定子痫前期组、子痫前期各个亚组及正常晚期妊娠组血清sEng及sFlt-1的水平。结果①子痫前期组孕妇血清sEng、sFlt-1水平分别为(5.27±1.05)ng/mL、(391.14±70.25)pg/mL,正常晚期妊娠组分别为(1.26±0.40)ng/mL、(27.57±4.36)pg/mL,两组比较差异有统计学意义(P<0.01);②轻度子痫前期组sEng水平与重度子痫前期组比较差异有统计学意义(P<0.01),两组sFlt-1水平比较差异无统计学意义(P>0.05);③早发型重度子痫前期组sEng水平与晚发型重度子痫前期组比较差异有统计学意义(P<0.01),两组sFlt-1水平比较差异无统计学意义(P>0.05)。结论子痫前期患者血清中sEngs、Flt-1水平显著升高,sEng在早发型重度子痫前期血清中升高更显著,对早期诊断子痫前期有重要意义。

Objective To investigate the expression and significance of serum soluble endoglin（sEng） and soluble fins-like tymsine kinase 1 （sFlt-1） in pre-eclampsia. Methods Serum levels of sEng 1 and sFlt-1 were determined by enzyme linked immunosorbent assay. Results The serum level of sEng of the pre-eclampsia group （5.27 ± 1.05）ng/mL was significantly higher than that of the controls [ （1.26 ± 0.40）ng/mL, P 〈 0.01 ], especially of early-onset severe pre-eclampsia. The serum level of sFlt-1 of the pre-eclampsia group （391.14 ±70.25）pg/mL was significantly higher than that of the third trimester group （27.57 ± 4.36）pg/mL, but sFlt-1 did not correlate with the most severe forms of the disease. Conclusion Serum levels of sEng and sFlt-1 are significantly increased in pre-eclampsia, and the serum level of sEng is even higher in severe pre-eclampsia patients.