肺干细胞标记物的研究与进展

Research and development of pulmonary stem cell markers

通过应用cells in induced group cultured for 2 weeks(von kossa staining)标记的胸腺嘧啶(3H-TdR)或5-溴脱氧尿核苷(BrdU)的渗入进行标记,发现肺组织的基底细胞、Clara细胞和肺泡II型上皮细胞可能具有干细胞特征,能最终分化形成气道上皮。目前对于肺干细胞的研究进展缓慢,究其原因主要是肺组织和气道具有复杂的内在结构,呼吸系统至少包含40种不同类型的细胞,呼吸上皮本身只有极低的生长更新率和非常有限的再生能力,同时肺干细胞的表面标志物也缺乏特异性,只能借助其他组织已知的干细胞标记物进行对比观察。对肺干细胞标记物的研究集中在Sca-1、ABCG2/Bcrp1、滞留细胞标记分子、细胞表面分子、细胞角蛋白、CCSP等方面。对肺癌发生的深入研究发现肺癌可能源于正常肺干细胞的突变,此对于肺癌的发生及治疗提供了更多的思路和方向。

By incorporation of tritiated thymidine or 5-bromodeoxyuridine and using them as the markers, we found that the basal cells, Clara cells and type Ⅱ alveolar epithelial cells in the pulmonary tissues may in some way behave like stem cells and can eventually differentiate into respiratory tract epithelium. The slow progress of researches on pulmonary stern cells up to date has its root in the complex structure of pulmonary tissues and respiratory tracts, which is demonstrated by the facts that the respiratory system is composed of at least 40 types of cells, plus that the growth, renewal, and regeneration rate or capacity of respiratory epithelium are extremely limited. Mean while, there have been no specific stem cell markers for pulmonary and lung cancer, so researchers rely on stem cell markers borrowed from other systems to search for stem cells in pulmonary system. At present, the studies of lung stem cell markers were focused on Sca-1, ABCG2/Bcrp 1, cell retaining marker, surface marker, cell keratin and CCSP Recently the lung stem cells have been shown to be the targets of transformation during lung carcinogenesis, which has provide more thoughts and direction for the occurrence and treatment of lung cancer.