人、鼠原肠形成蛋白基因的克隆和初步分析

Cloning and Preliminary Analysis of Twisted Gastrulation Protein Genes in Human and Mouse

目的:分析原肠形成蛋白(TSG)在几个发育阶段的人和小鼠睾丸中的差异表达。方法:应用cDNA微阵列技术对成人和胚胎睾丸进行基因表达图谱分析,获得在成人高表达、胚胎低表达的人TSG的全长基因;利用RT-PCR,从4周龄小鼠睾丸中克隆出小鼠TSG基因,用地高辛标记的探针进行原位杂交,检测小鼠TSG在睾丸中的表达。结果:人TSG在成人睾丸中高表达,在胚胎中低表达;小鼠TSG仅在小鼠睾丸生殖细胞中表达,在间质细胞中不表达。结论:小鼠睾丸TSG与骨形态发生蛋白(BMP)可能同步表达,提示在小鼠精子发生中也可能存在一条由TSG信号激活BMP的传导途径。

Objective： To investigate twisted gastrulation protein（TSG） in human and mouse testes on mRNA level in different developmental stage. Methods： Human full-length TSG（hTSG） eDNA was identified by microarray assay with eD- NA hybridization probes made from total mRNA of testes of adult and embryo. The mouse TSG full-length（mTSG） eDNA was cloned from mouse testes by RT-PCR, and mRNA of mTSG in testes was detected in four-week mouse testes by in situ hybridization. Results： mRNA of hTSG was found strongly expressed in adult testes, weakly in embryo testes, mTSG was found specifically expressed in germ cell. Conclusion： mTSG and bone morphogenetic proteins（BMP） were expressed in a similar pattern in testes, and TSG was closed related to BMP was proved before, all above suggested that pathway of BMP activated by TSG was associated with process of spermatogenesis.