牦牛EPOR基因编码区序列的克隆及分析

Molecular Cloning and Sequence Analysis of Yak Erythropoietin Receptor Gene

以处于不同海拔高度的3个牦牛品种即巴州牦牛、大通牦牛、九龙牦牛为研究对象,对各牦牛品种的促红细胞生成素受体(EPOR)基因编码区进行PCR扩增和克隆测序。在对测序结果进行拼接得到牦牛EPOR基因编码区全序列基础上,利用生物信息学软件对其序列进行生物信息学及比较基因组学分析。结果表明:牦牛的EPOR基因编码区全长1527bp,编码508个氨基酸;EPOR信号肽由25个氨基酸组成,胞外域由225个氨基酸组成,跨膜区由22个氨基酸组成,胞浆域由236个氨基酸组成;EPOR基因编码区在牦牛品种间及其与普通牛间均存在一定的差异,这些差异是否与牦牛的适应性有关,值得进一步研究。

Using the three yaks （Bazhou yak, Datong yak, Jiulong yak） which lived in the differnent altitudes as reaserch objects and the receptor for erythropoietin （EPO） gene was cloned by the PCR. The complete coding sequences （CDS） of the EPOR gene and deduced amino acid sequences of Bazhou yak, Datong yak and Jiulong yak were analyzed by the bio-information sofewares. The results showed that the length of CDS sequence of the EPOR gene in yak was 1 527bp and it encodes a 508-amino acid polypeptide chain. The yak EPOR had a signal peptide composed of 25 amino acids, extracellular domain composed of 225 amino acids, a single membran-spanning domain composed of 22 amino acids and intracellular domain composed of 236 amino acids. The differences of EPOR gene sequences not only existed among three yak breeds, hut also existed between the yak and cow. And whether these differences have relation with the extreme hypoxia environment of the plateau where the yaks live needs further research to prove it.