PTEN表达与胃癌预后因素分析

Clinical study of expression of PTEN in gastric cancer

目的:探讨胃癌组织中PTEN(张力蛋白和辅助蛋白同源、第10号染色体丢失的磷酸酶基因)和MMP-2(基质金属蛋白酶-2)蛋白表达与患者预后的关系。方法:用免疫组织化学S-P法检测64例胃癌组织PTEN和MMP-2蛋白表达,并对随访的33例胃癌患者进行单因素及多因素生存分析,选用Cox比例风险模型进行多因素生存分析。结果:PTEN蛋白在胃癌组织表达缺失率为21.98,其表达缺失与淋巴结转移正相关,与患者生存期负相关(P<0.05),Cox回归多因素生存分析显示PTEN蛋白表达缺失的患者生存的相对危险度是PTEN表达阳性的0.273倍(P<0.05);MMP-2蛋白在胃癌组织表达阳性率为68.75,其高表达与癌细胞浸润深度、淋巴结转移呈正相关,与生存期呈负相关(P<0.05)。另外,根治手术与患者的生存期呈正相关(P<0.05),非根治术患者生存的相对危险度是根治术患者的1.104倍(P<0.05)。结论:PTEN蛋白可作为胃癌患者预后的独立预测因子,PTEN蛋白表达缺失的胃癌患者预后不良。

Objective： To study the relationship of PTEN and MMP - 2 protein expression to gastric cancer progno- sis. Methods ： The expression of PTEN and MMP - 2 protein in 64 cases with gastric carcinoma was detected through immunohistochemistry S -P method, among them,33 patients were followed up and studied through multivariate analy- sis. Cox regression model was used for multivariate analysis. Results： The loss rate of PTEN protein was 21.98% in patients with gastric cancer, the loss rate had a positive correlation with lymph node metastasis, and a negative correla- tion with survival （ P 〈 0.05）, the survival analysis of Cox regression model revealed that the survival relative risk of patients without PTEN expression was 0. 273 times than those with PTEN expression （P 〈0.05）. The positive expres- sion rate of MMP -2 protein was 67.85% in the gastric cancer. The high expression of MMP -2 protein had a posi- tive correlation with infiltration level and lymph node metastasis,and a negative correlation with patients survival （P 〈 0.05 ）. Besides, radical operation had an obvious relationship with patients survival （P 〈 0.05）. The survival relative risk of patients with non - radical operation was 1. 104 times than those with radical operation. （ P 〈 0.05 ）. Conclusion： PTEN protein could be used as an independent factor for prognosis of gastric cancer, patients with loss of PTEN protein expression have bad prognosis.