肿瘤相关粘蛋白MUC1和抑癌基因p53在原发性肝癌中的表达

Expression of tumor-associated mucin MUC1 and p53 in primary liver carcinoma

目的:通过测定MUC1和p53在肝脏不同组织中的表达差异,探讨MUC1和p53在肿瘤诊断和免疫治疗中的意义。方法:采用免疫组化方法检测35例肝癌(28例肝细胞癌,7例胆管细胞癌),8例肝硬化,8例肝血管瘤和10例正常肝组织中MUC1和p53的表达。结果:MUC1和p53基因均阳性表达于肝癌组织,它们在肝癌组织中的阳性表达率明显高于其它肝脏组织(P<0.01),MUC1主要表达于细胞膜,其表达与肝癌的病理分型和组织学分化无相关性(P>0.05),但肝癌伴有门静脉癌栓组与不伴有门静脉癌栓组之间MUC1表达率具有显著差异性(P<0.05);p53表达于细胞核,在Ⅰ、Ⅱ、Ⅲ级肝癌组织中的表达呈现递增趋势。结论:p53与肝癌的发生发展密切相关,MUC1在肝癌组织中的表达与分布特点可以作为肝癌诊断、判断预后的指标,同时,MUC1作为一种靶抗原,为今后的肝癌免疫治疗提供新的线索。

Objective：To investigate the clinical significance of MUC1 and p53 in the diagnosis and immunothera- py of primary liver carcinoma. Methods： The expression of MUC1 and p53 were examined by immunohistoehemical analysis in 35 samples of liver carcinoma, including 28 hepatocellular carcinoma, 7 cholangio - carcinoma, 8 cirrhot- ic liver tissues; 8 samples of liver hemangioma and 10 samples of normal liver tissues. Results： Immunohistochemieal analysis showed over - expression of MUC1 and p53 in liver eaneinoma. The expression levels of MUC1 and p53 in liver carcinoma were higher than that of the other liver tissues （P 〈 0.01 ）. Expression of MUC1 was mainly localized in the cancer cell membrance. Expression level of MUC1 had no statistically correlation with pathological types and differentiation （P 〉 0.05）, but the expression level between with portal cancer emboli group and without portal cancer emboli group was significant difference（P 〈 0.05）. The expression level of t）53 in liver carcinoma was associated with stage Ⅰ ,Ⅱ and Ⅲ. Conclusion： p53 in is correlatied with the occurrence and development of liver carcinoma. Over - expression and locatization of MUC1 in primary liver carcinoma may act as diagnostic marker and pronogsis marker of liver carcinoma and mizht be as the tarzet of immunotheranv of liver carcinoma.