基质金属蛋白酶9基因多态性与2型糖尿病颈动脉粥样硬化的相关性

The Association Between Polymorphism of Matrix Metalloproteinase-9 Gene and Carotid Artery Arteriosclerosis in Type 2 Diabetes Mellitus

目的探讨基质金属蛋白酶9基因C1562T多态性与2型糖尿病患者颈动脉粥样硬化的关系。方法以中国广西桂林地区汉族人群158例为研究对象,包括60例健康对照者和98例2型糖尿病患者(其中非颈动脉粥样硬化者36例,合并颈动脉粥样硬化者62例),采用聚合酶链反应限制片长多态性分析检测其基因型,比较各组间基因型和等位基因频率,并分析其与2型糖尿病颈动脉粥样硬化的相关性。结果糖尿病组CT和TT基因型和T等位基因频率与对照组相比差异无显著性,糖尿病合并颈动脉粥样硬化组CT和TT基因型和T等位基因频率与糖尿病非颈动脉粥样硬化组相比显著升高(P<0.05);基质金属蛋白酶9T等位基因是2型糖尿病颈动脉粥样硬化的危险因素(OR=2.160,95%CI为1.026～4.547)。结论基质金属蛋白酶9基因C1562T多态性与2型糖尿病颈动脉粥样硬化有相关性。T等位基因可能是2型糖尿病颈动脉粥样硬化的易感基因。

Aim To investigate the association between a 1562C→T mutation of matrix metalloproteinase-9 （MMP-9） gene and carotid artery arteriosclerosis in type 2 diabetes mellitus （T2DM） of Guilin. Methods A case-control study for 158 Guilin Han Chinese subjects （including 98 type 2 diabetes mellitus and 60 normal control） was performed. The number of the 1562 C→T mutation alleles were determined by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method. Results The frequencies of genotype and allele showed no significant difference between control groups and T2DM group, and the frequency of the T genotype and T allele at 1562 were significantly higher in T2DM with carotid artery arteriosclerosis group than in T2DM without carotid artery arteriosclerosis group and control subjects （P〈0.05）. The analysis showed that MMP-9 T allele was an important risk factor of carotid artery arteriosclerosis in T2DM（OR=2.160, 95%CI：1.026～4.547）. Conclusion The T allele of C1562T polymorphism of MMP-9 gene is related to carotid artery arteriosclerosis in Guilin T2DM. The T allele may be a risk factor.