广东省2007年度非伤寒沙门菌监测及病原学特征分析

Surveillance and pathogenic analysis on non-typhoidal Salmonella in Guangdong province, 2007

目的了解广东省腹泻病患者中非伤寒沙门菌感染情况和菌株的血清型别、分布、耐药性变化。方法对纳入研究的腹泻病患者进行非伤寒沙门菌检测，对分离到的菌株进行血清分型、药物敏感性试验和脉冲场凝胶电泳（PFGE）分型。结果2007年度共检测1128份腹泻粪便标本，分离到71株沙门菌，阳性检出率为6．29％；共分得29种血清型，肠炎和鼠伤寒沙门菌居多；大多数沙门菌对常用的头孢类和喹诺酮类抗生素敏感，鼠伤寒沙门菌的耐药率普遍较肠炎和斯坦利沙门菌高；除肠炎沙门菌外，其余的血清型没有同一PFGE型别的菌株；用XbaⅠ酶切17株肠炎沙门菌可分为PFGE—XbaⅠ1～8型，其中PFGE—XbaⅠ4型为优势型别。用SfiⅠ和NotⅠ酶对12株肠炎沙门菌进行再分型，综合用XbaⅠ／SfiⅠ／NotⅠ三种酶的结果发现仍有三组菌的PFGE图谱是完全一致的。结论2007年度广东省非伤寒沙门菌的感染多数为散发病例，头孢类和喹诺酮类抗生素是治疗非伤寒沙门菌感染的首选药物。

Objective To understand non-typhoid Salmonella in diarrhea patients from Guangdong province in order to timely discover the outbreaks caused by them as well as to grasp the serotypes, antibiotic resistance and pulse-field gel eleetrophoresis （PFGE） types of those strains isolated from this surveillance program. Methods Salmonella strains from diarrhea patients were detected and all the positive strains were tested by serum agglutination, antibiotic susceptibility and PFGE. Results 71 non- typhoid Salmonella strains were isolated from 1128 stoop samples, with a positive rate of 6.29 %. All the strains were divided into 29 serotypes, with Salmonella serotype enteritidis and typhimurium showing the most common serotypes. Most of the strains were sensitive to cephalosporins and quinolones. The antibiotic resistance rates of S. typhimurium were higher than S.enteritidis and S.stanley. Other than S. enteritidis, all the serotype strains did not have the same type of PFGE. 17 S. enteritidis strains digested by Xba Ⅰ were divided to 8 PFGE types while the PFGE 4 type appeared the most common one. 12 S. enteritidis strains were typed again by Sfi Ⅰ and Not Ⅰ , and there were still 3 groups of strains showing the same PFGE pattern. Conclusion Most of the infection caused by non-typhoidal Salmonella was sporadic in Guangdong province in 2007. Cephalosporins and quinolones seemed the best in curing the infection of non-typhoidal Salmonella at the clinics.