丙氨酰谷氨酰胺对断奶仔猪生长和血清抗氧化能力的影响

Effects of Alanyl-glutamine on Growth and Serum Antioxidant Ability of Weaned Piglets

本试验旨在研究丙氨酰谷氨酰胺（Ala—Gln）对断奶仔猪生长及抗氧化能力的影响。试验选择经产母猪产的大x长二元杂交仔猪48头（公母各占1／2），21d断奶，按公母比例一致，同窝次均分的原则，随机分成4个处理，每个处理12个重复，每个重复1头猪。A组为对照组，饲喂基础日粮；B、C和D组为试验组，分别饲喂在基础日粮中添加0．1％、0．3％和0．5％Ala—Gin的Et粮。试验结果表明，随日粮Ala—Gin添加水平提高，21～31d时，仔猪的平均日增重以二次曲线方式提高（P=0．04），31～41d和21-41d时，仔猪的平均日增重均以线性方式提高（P=0．07和P=0．05）；31d时，添加0．5％的Ala—Gin显著降低仔猪血清尿素氮（BUN）含量（P〈0．05），且随着日粮Ala—Gin添加水平提高，仔猪BUN含量以线性方式降低（P=0．06）；添加Ala—Gin对仔猪血清过氧化氢酶（cAT）、谷胱甘肽过氧化物酶（GSH-Px）活力、总抗氧化能力（T—AOC）和血清丙二醛（MDA）含量均有不同程度的影响，其中添加0．1％、0．3％和0．5％的Ala-Gin均显著提高仔猪血清GSH-Px活力（P〈0．05），且在31和41d时，血清GSH—Px活力以线性方式升高（P=0．01和P=0．05）。结果提示，断奶仔猪日粮中添加Ala—Gin能促进体内蛋白质合成，提高血清抗氧化能力，促进仔猪生长。

This trial was conducted to study the effects of alanyl-glutamine （Ala-Gln） on growth and serum antioxidant ability of weaned piglets. Forty eight crossbred Landrace x Yorkshire weaned piglets （half male, half female） at the age of 21 days. were selected and randomly allocate to 4 treatment groups with 12 replicates each. Group A （control） was fed the basal diet and group B, C and D as three trial groups were fed the basal diet added 0.1%, 0.3% and 0.5% Ala-Gln, respectively. The results showed that average daily gain significantly increased in a quadratic fashion （quadratic, P = 0.04） at the age of 21-31 days and increased by linear form （linear, P = 0.07 and P = 0.05） at the age of 31-41 days and 21-41 days with Ala-Gln supplementation levels increasing. Level of serum urea nitrogen （BUN） significantly decreased （P〈0.05） with adding 0.5% Ala-Gln, and level of BUN decreased （linear, P = 0.06） at the age of 31 days with Ala-Gln supplementation levels increasing. Serum catalase （CAT）, glutathione peroxidase（GSH-Px） activity,total antioxidant capacity （T-AOC） and level of serum malondialdehyde （MDA） were affected by adding Ala-Gln. GSH-Px activity significantly increased （P〈 0.05） with adding 0.1%, 0.3% and 0.5% Ala-Gln. With Ala-Gln supplementation levels increasing, serum GSH-Px activity increased （linear, P = 0.01 and P = 0.05） at the age of 31 and 41 days. In conclusion, addition Ala-Gln could improve protein synthesis and growth performance, and increase serum antioxidant ability in weaned piglets.